Ekström Simon, Wallman Lars, Malm Johan, Becker Charlotte, Lilja Hans, Laurell Thomas, Marko-Varga György
Department of Electrical Measurements, Lund Institute of Technology, Lund, Sweden.
Electrophoresis. 2004 Nov;25(21-22):3769-77. doi: 10.1002/elps.200406094.
The performance of a miniaturized sample processing platform for matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), manufactured by silicon microfabrication, called integrated selective enrichment target (ISET) technology was evaluated in a biological context. The ISET serves as both sample treatment device and MALDI-MS target, and contains an array of 96 perforated nanovials, which each can be filled with 40 nL of reversed-phase beads. This methodology minimizes the number of sample transfers and the total surface area available for undesired adsorption of the analytes in order to provide high-sensitivity analysis. ISET technology was successfully applied for characterization of proteins coisolated by affinity chromatography of prostate-specific antigen (PSA) from human seminal fluid. The application of ISET sample preparation enabled multiple analyses to be performed on a limited sample volume, which resulted in the discovery that prolactin inducible protein (PIP) was coisolated from the samples.
对一种通过硅微加工制造的用于基质辅助激光解吸/电离质谱（MALDI-MS）的小型化样品处理平台——集成选择性富集靶标（ISET）技术在生物学背景下进行了评估。ISET既作为样品处理装置又作为MALDI-MS靶标，包含一个由96个穿孔纳米小瓶组成的阵列，每个小瓶可填充40 nL反相珠。这种方法最大限度地减少了样品转移的次数以及分析物非预期吸附的总表面积，以提供高灵敏度分析。ISET技术已成功应用于通过亲和色谱法从人类精液中共分离前列腺特异性抗原（PSA）的蛋白质的表征。ISET样品制备的应用使得能够在有限的样品体积上进行多次分析，结果发现催乳素诱导蛋白（PIP）从样品中被共分离出来。
