Tu Tingting, Gross Michael L
Center for Biomedical and Bioorganic Mass Spectrometry, Department of Chemistry, Washington University in St. Louis, St. Louis, MO 63130, USA.
Trends Analyt Chem. 2009 Aug 1;28(7):833-841. doi: 10.1016/j.trac.2009.03.013.
The trend of miniaturization in bioanalytical chemistry is shifting from technical development to practical application. In matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS), progress in miniaturizing sample spots has been driven by the needs to increase sensitivity and speed, to interface with other analytical microtechnologies, and to develop miniaturized instrumentation.We review recent developments in miniaturizing sample spots for MALDI-MS. We cover both target modification and microdispensing technologies, and we emphasize the benefits with respect to sensitivity, throughput and automation.We hope that this review will encourage further method development and application of miniaturized sample spots for MALDI-MS, so as to expand applications in analytical chemistry, protein science and molecular biology.
生物分析化学中的小型化趋势正从技术开发转向实际应用。在基质辅助激光解吸/电离质谱（MALDI-MS）中，样品斑点小型化的进展是由提高灵敏度和速度、与其他分析微技术联用以及开发小型化仪器的需求所推动的。我们综述了MALDI-MS样品斑点小型化的最新进展。我们涵盖了靶材修饰和微量分配技术，并强调了其在灵敏度、通量和自动化方面的优势。我们希望这篇综述将鼓励进一步开发用于MALDI-MS的小型化样品斑点方法并加以应用，从而扩大其在分析化学、蛋白质科学和分子生物学中的应用。
