Maarse A C, de Haan M, Bout A, Grivell L A
Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Nucleic Acids Res. 1988 Jul 11;16(13):5797-811. doi: 10.1093/nar/16.13.5797.
A regulatory element has been identified in the promoter region of the gene encoding the 11 kDa subunit VIII of the ubiquinol-cytochrome c oxidoreductase in Saccharomyces cerevisiae. The element, which is approximately 40 bp long and situated 185 bp upstream of the initiator ATG, is essential for induction of gene expression during growth in the presence of non-fermentable carbon sources. This is shown by the regulated synthesis of beta-galactosidase in yeast cells harbouring a CYC1-lacZ fusion gene, in which the CYC1 UAS's had been replaced by a 43 bp subunit VIII gene promoter fragment. In addition two DNA-binding activities, which may represent either separate factors or different forms of a single factor, have been detected. Both factors are abundant and they bind in a mutually exclusive fashion to a DNA sequence just upstream of the regulatory element. Although it is unlikely that these factors are directly involved in the response of the subunit VIII gene to catabolite repression, the position of their binding sites relative to the UAS and to the 3'-terminus of a gene located only 361 bp upstream suggest that they are important in modulating transcriptional activity of this region.
在酿酒酵母中，泛醇 - 细胞色素c氧化还原酶11 kDa亚基VIII编码基因的启动子区域已鉴定出一种调控元件。该元件长度约为40 bp，位于起始密码子ATG上游185 bp处，对于在非发酵碳源存在下生长期间基因表达的诱导至关重要。这通过在含有CYC1 - lacZ融合基因的酵母细胞中β - 半乳糖苷酶的调控合成得以证明，其中CYC1的上游激活序列（UAS）已被一个43 bp的亚基VIII基因启动子片段所取代。此外，还检测到两种DNA结合活性，它们可能代表不同的因子或单一因子的不同形式。这两种因子都很丰富，并且它们以相互排斥的方式结合到调控元件上游的一个DNA序列上。尽管这些因子不太可能直接参与亚基VIII基因对分解代谢物阻遏的反应，但它们结合位点相对于UAS以及位于仅上游361 bp处一个基因的3'末端的位置表明，它们在调节该区域的转录活性方面很重要。
