Panzeri L, Philippsen P
EMBO J. 1982;1(12):1605-11. doi: 10.1002/j.1460-2075.1982.tb01362.x.
The functional sequence from the centromere in chromosome VI ( CEN6 ) of Saccharomyces cerevisiae was narrowed down to a stretch of 500 bp by a Bal31 deletion approach. The DNA sequence in this region shows three long stretches, 40 bp, 96 bp, and 63 bp of 85% and more AT pairs and a pyrimidine purine bias in the individual single strands. We assume that the CEN6 functional sequences encompass these AT-rich stretches because this part shows striking similarities to sequence elements common to CEN3 and CEN11 DNA. A strain comparison revealed that CEN6 DNA sequences are confined to the Saccharomyces genus and probably only to the S. cerevisiae species. CEN6 is not highly conserved within S. cerevisiae strains because EcoRI and HindIII restriction site variants are found with high frequency.
通过Bal31缺失方法，酿酒酵母第六条染色体（CEN6）着丝粒的功能序列被缩小到一段500 bp的区域。该区域的DNA序列显示出三个长片段，分别为40 bp、96 bp和63 bp，其中85%及以上为AT碱基对，且单链中存在嘧啶嘌呤偏好。我们推测CEN6功能序列包含这些富含AT的片段，因为这部分与CEN3和CEN11 DNA的共同序列元件具有显著相似性。菌株比较表明，CEN6 DNA序列局限于酿酒酵母属，可能仅存在于酿酒酵母物种中。CEN6在酿酒酵母菌株中并非高度保守，因为EcoRI和HindIII限制酶切位点变体的出现频率很高。
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