整合宿主因子（IHF）与大肠杆菌K12的ilvGMEDA操纵子的ilvGp1启动子的结合。
Binding of integration host factor (IHF) to the ilvGp1 promoter of the ilvGMEDA operon of Escherichia coli K12.
作者信息
Pereira R F, Ortuno M J, Lawther R P
机构信息
Department of Biology, University of South Carolina, Columbia.
出版信息
Nucleic Acids Res. 1988 Jul 11;16(13):5973-89. doi: 10.1093/nar/16.13.5973.
Crude extracts of Escherichia coli K-12 were found to bind DNA restriction fragments containing ilvGp1. Our analysis using a series of restriction fragments and a BamHI linker mutation indicate that a factor binds to ilvGp1 or adjacent to it. Analysis with mutant strains of E. coli K-12 and purified IHF indicate that IHF binds to ilvGp1. Furthermore, both analysis in vivo and in vitro indicate that IHF precludes transcription from ilvGp1.
研究发现大肠杆菌K-12的粗提物能结合含有ilvGp1的DNA限制片段。我们使用一系列限制片段和一个BamHI接头突变进行的分析表明，有一种因子能结合到ilvGp1或其附近。对大肠杆菌K-12突变菌株和纯化的整合宿主因子（IHF）进行的分析表明，IHF能结合到ilvGp1上。此外，体内和体外分析均表明，IHF会阻止ilvGp1的转录。
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