Razzaque A, Jahan N, McWeeney D, Jariwalla R J, Jones C, Brady J, Rosenthal L J
Department of Microbiology, Georgetown Medical Center, Washington, DC 20007.
Proc Natl Acad Sci U S A. 1988 Aug;85(15):5709-13. doi: 10.1073/pnas.85.15.5709.
To define the morphological transforming region II (mtrII) of human cytomegalovirus (HCMV), a series of subclones of the Xba I/BamHI fragment EM was constructed in vitro and tested for focus-forming activity and tumorigenicity. A 980-base-pair subclone of fragment EM was identified, and its nucleotide sequence revealed three small open reading frames (ORFs), encoding 79, 83, and 34 amino acid residues. S1 nuclease analysis of HCMV-infected cells identified several distinct early RNA species within mtrII, two of which (P1 and P2) were of particular interest, since the length of the protected DNA fragments would position the 5' end of the RNAs upstream of the open reading frames. In addition, the 980-base-pair transforming sequence revealed DNA elements capable of forming stem-loop structures. Thus the transforming mtrII domain of HCMV strain Towne contains both small open reading frames that are expressed in lytically infected cells and sequences resembling insertion-like structures that may be involved in transformation.
为了确定人巨细胞病毒（HCMV）的形态转化区域II（mtrII），体外构建了Xba I/BamHI片段EM的一系列亚克隆，并检测其集落形成活性和致瘤性。鉴定出片段EM的一个980碱基对的亚克隆，其核苷酸序列显示有三个小的开放阅读框（ORF），分别编码79、83和34个氨基酸残基。对HCMV感染细胞进行S1核酸酶分析，在mtrII内鉴定出几种不同的早期RNA种类，其中两种（P1和P2）特别令人感兴趣，因为受保护的DNA片段长度会将RNA的5'端定位在开放阅读框的上游。此外，980碱基对的转化序列显示出能够形成茎环结构的DNA元件。因此，HCMV毒株汤氏株的转化mtrII结构域既包含在裂解感染细胞中表达的小开放阅读框，也包含可能参与转化的类似插入样结构的序列。
