具有转化活性的疱疹病毒DNA小片段含有类插入序列结构。
Small fragments of herpesvirus DNA with transforming activity contain insertion sequence-like structures.
作者信息
Galloway D A, Nelson J A, McDougall J K
出版信息
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4736-40. doi: 10.1073/pnas.81.15.4736.
Abstract
A 737-base-pair fragment of herpes simplex virus type 2 DNA with morphological-transforming ability was identified by transfecting into rodent cells deleted fragments of the left-hand end of the Bgl II N fragment region (map position 0.58-0.625), which were constructed in vitro. The transforming sequences lie within the coding region for a Mr 61,000 protein, but the fragment itself does not appear to specify a viral polypeptide. Contained within the transforming fragment are sequences that can be drawn as a stem-loop structure flanked by direct repeats, similar to an insertion sequence-like element. An insertion sequence-like structure was also found in a small fragment of human cytomegalovirus DNA that has transforming activity. Possible mechanisms of herpesvirus transformation are discussed.
摘要
通过将体外构建的Bgl II N片段区域（图谱位置0.58 - 0.625）左手端的缺失片段转染到啮齿动物细胞中，鉴定出了具有形态转化能力的单纯疱疹病毒2型DNA的一个737个碱基对的片段。转化序列位于一种分子量为61,000的蛋白质的编码区内，但该片段本身似乎并不编码病毒多肽。转化片段中包含一些序列，这些序列可以绘制成一个由正向重复序列侧翼的茎环结构，类似于一个插入序列样元件。在具有转化活性的人巨细胞病毒DNA的一个小片段中也发现了一个插入序列样结构。文中讨论了疱疹病毒转化的可能机制。
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