人巨细胞病毒汤氏株中形态转化区域III的定位与序列分析

Localization and sequence analysis of morphological transforming region III within human cytomegalovirus strain Towne.

作者信息

Thompson J, Inamdar A, Jahan N, Doniger J, Rosenthal L J

机构信息

Department of Microbiology and Immunology, Georgetown University School of Medicine, Washington, D.C. 20007-2197.

出版信息

Intervirology. 1993;36(3):121-7. doi: 10.1159/000150330.

DOI:10.1159/000150330

PMID:8150593

Abstract

A 7.6-kbp BamHI/XbaI EJ subfragment of the Towne XbaI-E fragment of human cytomegalovirus (HCMV) strain Towne has been previously designated as morphological transforming region III (mtrIII) because it induced focal and tumorigenic transformation of rodent fibroblasts. However, in two separate cell systems, mtrIII sequences were not retained because they were not detected in either the focal, tumor or tumor-derived cell lines. In this report, mtrIII was localized to a 2.1-kbp SalI/XbaI DNA fragment. The sequence of the 2,085-bp region was determined and compared to the colinear DNA from HCMV strain AD169. DNA sequence analysis revealed the presence of five open reading frames in Towne mtrIII, two of which are conserved in strain AD169. The localization and sequence analysis of mtrIII will allow further investigation as to the mechanism(s) by which HCMV may play a role in human cancer.

摘要

人巨细胞病毒（HCMV）毒株汤氏株（Towne）的汤氏株XbaI-E片段的一个7.6千碱基对的BamHI/XbaI EJ亚片段先前被指定为形态转化区域III（mtrIII），因为它能诱导啮齿动物成纤维细胞发生灶性和致瘤性转化。然而，在两个不同的细胞系统中，mtrIII序列并未保留，因为在灶性、肿瘤或肿瘤衍生的细胞系中均未检测到它们。在本报告中，mtrIII被定位到一个2.1千碱基对的SalI/XbaI DNA片段上。测定了该2085碱基对区域的序列，并与HCMV毒株AD169的共线DNA进行了比较。DNA序列分析显示汤氏株mtrIII中存在五个开放阅读框，其中两个在AD169毒株中是保守的。mtrIII的定位和序列分析将有助于进一步研究HCMV可能在人类癌症中发挥作用的机制。