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Characterization of single-copy probe from vicinity of centromere of human chromosome 1.

作者信息

Solus J, Jacquemin-Sablon A, Carine K, Waltzer E, Scheffler I E

机构信息

Department of Biology, University of California, San Diego, La Jolla 92093.

出版信息

Somat Cell Mol Genet. 1988 Jul;14(4):381-91. doi: 10.1007/BF01534646.

DOI:10.1007/BF01534646
PMID:2840744
Abstract

Human DNA sequences in the human-hamster somatic cell hybrid XJM12.1.3 exist in the form of a minichromosome including the centromere of human chromosome 1. We describe the cloning of XJM12.1.3 DNA into the lambda vector EMBL3, the identification of minichromosome DNA-containing recombinants by hybridization with human sequences, and the characterization of one recombinant as a specific and unique probe for a region close to the centromere of human chromosome 1. This probe and others isolated from the minichromosome DNA are being developed to permit molecular access to a human centromere and its functional sequences.

摘要

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