Fatih Aydın Abdurrahman, Küçükgergin Canan, Bingül İlknur, Doğan-Ekici Işın, Doğru-Abbasoğlu Semra, Uysal Müjdat
Department of Biochemistry, Istanbul Medical Faculty, Istanbul University, Istanbul, Turkey.
Department of Pathology, Yeditepe University Medical Faculty, Kayışdağı, Istanbul, Turkey.
Exp Clin Endocrinol Diabetes. 2017 May;125(5):282-289. doi: 10.1055/s-0043-100117. Epub 2017 Apr 13.
High fat diet (HFD) and low dose of streptozotocin (STZ)-treated rats provide an animal model for type 2 Diabetes Mellitus (T2DM). Oxidative stress plays a role in the development of diabetic complications. Carnosine (CAR) has antioxidant and antiglycating properties. We investigated effects of CAR on renal function, oxidation and glycation products in HFD+STZ-rats. Rats were fed with HFD (60% of total calories from fat) for 4 weeks and then a single dose STZ (40 mg/kg; i.p.) was applied. Rats with blood glucose levels above 200 mg/dL were fed with HFD until the end of the 12 week. CAR (250 mg/kg body weight; i.p.; 5 times a week) was administered to rats for the last 4 weeks. Glycated hemoglobin (HbA1c), glucose, lipids, and andrenal function tests in serum as well as reactive oxygen species, malondialdehyde, protein carbonyl, advanced oxidation protein products, advanced glycation end products (AGEs), antioxidant power, and antioxidant enzyme activities and their mRNA expressions in kidneys were determined. CAR treatment did not alter glucose and HbA1c, but it decreased serum lipids, creatinine, and urea levels in HFD+STZ rats. Oxidation products of lipids and proteins and AGEs levels decreased, but antioxidant enzyme activities and their mRNA expressions remained unchanged due to CAR treatment. Our results indicate that CAR treatment alleviated renal function and decreased accumulation of oxidation and glycation products in kidneys in HFD+STZ-rats.
高脂饮食（HFD）和低剂量链脲佐菌素（STZ）处理的大鼠可作为2型糖尿病（T2DM）的动物模型。氧化应激在糖尿病并发症的发生发展中起作用。肌肽（CAR）具有抗氧化和抗糖化特性。我们研究了CAR对HFD + STZ大鼠肾功能、氧化和糖化产物的影响。大鼠喂食HFD（脂肪提供总热量的60%）4周，然后单次腹腔注射STZ（40 mg/kg）。血糖水平高于200 mg/dL的大鼠继续喂食HFD直至12周结束。在最后4周，给大鼠腹腔注射CAR（250 mg/kg体重，每周5次）。测定血清中的糖化血红蛋白（HbA1c）、葡萄糖、脂质和肾功能指标，以及肾脏中的活性氧、丙二醛、蛋白质羰基、晚期氧化蛋白产物、晚期糖基化终产物（AGEs）、抗氧化能力、抗氧化酶活性及其mRNA表达。CAR治疗未改变葡萄糖和HbA1c水平，但降低了HFD + STZ大鼠的血清脂质、肌酐和尿素水平。脂质和蛋白质的氧化产物以及AGEs水平降低，但CAR治疗后抗氧化酶活性及其mRNA表达未改变。我们的结果表明，CAR治疗可改善HFD + STZ大鼠的肾功能，并减少肾脏中氧化和糖化产物的积累。
