Čabanová Viktória, Kocák Peter, Víchová Bronislava, Miterpáková Martina
Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01, Košice, Slovakia.
Private Veterinary Practice, Pekárenská 1, 071 01, Michalovce, Slovakia.
Parasit Vectors. 2017 Apr 13;10(1):179. doi: 10.1186/s13071-017-2128-2.
The spirurid nematode Thelazia callipaeda, also called the "Oriental eyeworm", is the causative agent of canine and human ocular thelaziosis. In the past few years it has started to spread across central Europe and new endemic areas have been established. The present study reports on the first four autochthonous cases of canine ocular thelaziosis in the territory of Slovakia, Central Europe.
All cases were recorded in dogs living in eastern Slovakia, near the border with the Ukraine. All worms collected were investigated morphologically and their identification further confirmed at the molecular level by PCR amplification and direct sequencing. Nucleotide sequences of partial T. callipaeda cox1 and 28S rDNA gene fragments isolated from Slovak dogs were submitted to the GenBank database under accession numbers KY476400 and KY476401, respectively.
Considering that all four cases were diagnosed in animals that had never travelled abroad, there is clear evidence of an autochthonous occurrence and thereby the further spread of T. callipaeda across Europe. Moreover, at latitude of 48°N, these cases might be considered as the northernmost recorded cases of autochthonous in western and Central Europe.
旋尾线虫结膜吸吮线虫，也被称为“东方眼虫”，是犬类和人类眼结膜吸吮线虫病的病原体。在过去几年中，它已开始在中欧传播，并建立了新的流行地区。本研究报告了中欧斯洛伐克境内首例4例犬类眼结膜吸吮线虫病本土病例。
所有病例均记录在生活在斯洛伐克东部、靠近乌克兰边境的犬只身上。对所有采集到的蠕虫进行了形态学研究，并通过PCR扩增和直接测序在分子水平上进一步确认了它们的身份。从斯洛伐克犬只中分离出的结膜吸吮线虫部分cox1和28S rDNA基因片段的核苷酸序列分别以登录号KY476400和KY476401提交到GenBank数据库。
鉴于所有4例病例均在从未出过国的动物中被诊断出来，有明确证据表明结膜吸吮线虫在本土出现，从而在欧洲进一步传播。此外，在北纬48°的地区，这些病例可能被视为西欧和中欧有记录的最北部本土病例。
