Limchoowong Nunticha, Sricharoen Phitchan, Techawongstien Suchila, Chanthai Saksit
Materials Chemistry Research Unit, Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand.
Food Chem. 2017 Sep 1;230:398-404. doi: 10.1016/j.foodchem.2017.03.070. Epub 2017 Mar 14.
A green approach using chitosan solution as a novel bio-dispersive agent for the dispersive liquid-liquid microextraction (DLLME) of trace amounts of Cu(II) in edible oils is presented. An emulsion was formed by mixing the oil sample with 300µL of 0.25% (w/v) chitosan solution containing 200µL of 6molL HCl. Deionized water was used to induce emulsion breaking without centrifugation. The centrifuged Cu(II) extract was collected and analyzed using an inductively coupled plasma-optical emission spectrometer. The detection and quantitation limits were 2.1 and 6.8µgL, respectively. Trace amounts of Cu(II) in six edible oil samples were tested under optimum conditions for DLLME, with a recovery ranging from 90.3% to 109.3%. Therefore, the new dispersive agent in DLLME offers superior performance owing to the non-toxic nature of the solvent, short extraction time, high sensitivity, and easy operation.
本文提出了一种绿色方法，使用壳聚糖溶液作为新型生物分散剂，用于食用油中痕量铜（II）的分散液液微萃取（DLLME）。将油样与300µL含200µL 6mol/L盐酸的0.25%（w/v）壳聚糖溶液混合形成乳液。使用去离子水诱导乳液破乳，无需离心。收集离心后的铜（II）萃取物，并用电感耦合等离子体发射光谱仪进行分析。检测限和定量限分别为2.1和6.8µg/L。在DLLME的最佳条件下对六个食用油样品中的痕量铜（II）进行了测试，回收率在90.3%至109.3%之间。因此，DLLME中的新型分散剂由于溶剂无毒、萃取时间短、灵敏度高且操作简便，具有卓越的性能。
