Decambron A, Manassero M, Bensidhoum M, Lecuelle B, Logeart-Avramoglou D, Petite H, Viateau V
Laboratory of Bioengineering and Bioimaging for Osteo-Articular tissues (B2OA), 10 Avenue de Verdun, 75010 Paris and Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France
Laboratory of Bioengineering and Bioimaging for Osteo-Articular tissues (B2OA), 10 Avenue de Verdun, 75010 Paris and Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 Avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France.
Bone Joint Res. 2017 Apr;6(4):208-215. doi: 10.1302/2046-3758.64.BJR-2016-0236.R1.
To compare the therapeutic potential of tissue-engineered constructs (TECs) combining mesenchymal stem cells (MSCs) and coral granules from either or to repair large bone defects.
Bone marrow-derived, autologous MSCs were seeded on or coral granules in a perfusion bioreactor. -TECs (n = 7), -TECs (n = 6) and bone autografts (n = 2) were then implanted into 25 mm long metatarsal diaphyseal defects in sheep. Bimonthly radiographic follow-up was completed until killing four months post-operatively. Explants were subsequently processed for microCT and histology to assess bone formation and coral bioresorption. Statistical analyses comprised Mann-Whitney, -test and Kruskal-Wallis tests. Data were expressed as mean and standard deviation.
A two-fold increaseof newly formed bone volume was observed for -TECs when compared with -TECs (14 sd 1089 mm 782 sd 507 mm; p = 0.09). Bone union was consistent with autograft (1960 sd 518 mm). The kinetics of bioresorption and bioresorption rates at four months were different for -TECs and -TECs (81% sd 5% 94% sd 6%; p = 0.04). In comparing the defects that healed with those that did not, we observed that, when major bioresorption of coral at two months occurs and a scaffold material bioresorption rate superior to 90% at four months is achieved, bone nonunion consistently occurred using coral-based TECs.
Bone regeneration in critical-size defects could be obtained with full bioresorption of the scaffold using coral-based TECs in a large animal model. The superior performance of -TECs brings us closer to a clinical application, probably because of more suitable bioresorption kinetics. However, nonunion still occurred in nearly half of the bone defects. A. Decambron, M. Manassero, M. Bensidhoum, B. Lecuelle, D. Logeart-Avramoglou, H. Petite, V. Viateau. A comparative study of tissue-engineered constructs from and coral in a large animal bone defect model. 2017;6:208-215. DOI: 10.1302/2046-3758.64.BJR-2016-0236.R1.
比较结合间充质干细胞（MSCs）和来自[具体来源1]或[具体来源2]的珊瑚颗粒的组织工程构建体（TECs）修复大骨缺损的治疗潜力。
将骨髓来源的自体MSCs接种到灌注生物反应器中的[具体来源1]或[具体来源2]珊瑚颗粒上。然后将[来源1] - TECs（n = 7）、[来源2] - TECs（n = 6）和自体骨移植（n = 2）植入绵羊25毫米长的跖骨干骺端缺损处。术后每两个月进行一次放射学随访，直至术后四个月处死动物。随后对取出的样本进行显微CT和组织学处理，以评估骨形成和珊瑚生物吸收情况。统计分析包括Mann - Whitney检验、t检验和Kruskal - Wallis检验。数据以平均值和标准差表示。
与[来源2] - TECs相比，[来源1] - TECs新形成的骨体积增加了两倍（14±1089立方毫米对78±507立方毫米；p = 0.09）。骨愈合情况与自体骨移植一致（196±518立方毫米）。[来源1] - TECs和[来源2] - TECs在四个月时的生物吸收动力学和生物吸收率不同（81%±5%对94%±6%；p = 0.04）。在比较愈合和未愈合的缺损时，我们观察到，当两个月时珊瑚发生主要生物吸收且四个月时支架材料生物吸收率超过90%时，使用基于珊瑚的TECs会持续出现骨不连。
在大型动物模型中，使用基于珊瑚的TECs使支架完全生物吸收可实现临界尺寸缺损的骨再生。[来源1] - TECs的优越性能使我们更接近临床应用，可能是因为其生物吸收动力学更合适。然而，近一半的骨缺损仍发生骨不连。A. 德坎布隆、M. 马纳塞罗、M. 本西杜姆、B. 勒屈埃尔、D. 洛热尔 - 阿夫拉莫格鲁、H. 珀蒂特、V. 维亚托。大型动物骨缺损模型中来自[具体来源1]和[具体来源2]珊瑚的组织工程构建体的比较研究。《骨与关节研究》2017年；6卷：208 - 215页。DOI: 10.1302/2046 - 3758.64.BJR - 2016 - 0236.R1 。
