Department of Trauma, Hand and Reconstructive Surgery, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Center of Physiology, Cardiovascular Physiology, Goethe University Frankfurt, 60590 Frankfurt am Main, Germany.
Cells. 2021 May 19;10(5):1249. doi: 10.3390/cells10051249.
Regeneration of large bone defects is a major objective in trauma surgery. Bone marrow mononuclear cell (BMC)-supported bone healing was shown to be efficient after immobilization on a scaffold. We hypothesized that fibrous demineralized bone matrix (DBM) in various forms with BMCs is superior to granular DBM. A total of 65 male SD rats were assigned to five treatment groups: syngenic cancellous bone (SCB), fibrous demineralized bone matrix (f-DBM), fibrous demineralized bone matrix densely packed (f-DBM 120%), DBM granules (GDBM) and DBM granules 5% calcium phosphate (GDBM5%Ca2). BMCs from donor rats were combined with different scaffolds and placed into 5 mm femoral bone defects. After 8 weeks, bone mineral density (BMD), biomechanical stability and histology were assessed. Similar biomechanical properties of f-DBM and SCB defects were observed. Similar bone and cartilage formation was found in all groups, but a significantly bigger residual defect size was found in GDBM. High bone healing scores were found in f-DBM (25) and SCB (25). The application of DBM in fiber form combined with the application of BMCs shows promising results comparable to the gold standard, syngenic cancellous bone. Denser packing of fibers or higher amount of calcium phosphate has no positive effect.
大骨缺损的再生是创伤外科的主要目标。研究表明，在支架上固定骨髓单核细胞（BMC）支持的骨愈合是有效的。我们假设，用 BMC 支持的各种形式的纤维脱矿骨基质（DBM）优于颗粒状 DBM。总共 65 只雄性 SD 大鼠被分为五组治疗：同种异体松质骨（SCB）、纤维脱矿骨基质（f-DBM）、紧密堆积的纤维脱矿骨基质（f-DBM120%）、DBM 颗粒（GDBM）和 DBM 颗粒 5%磷酸钙（GDBM5%Ca2）。供体大鼠的 BMC 与不同的支架结合，并放置到 5mm 股骨缺损中。8 周后，评估骨矿物质密度（BMD）、生物力学稳定性和组织学。观察到 f-DBM 和 SCB 缺陷具有相似的生物力学特性。所有组都发现了相似的骨和软骨形成，但在 GDBM 中发现了明显更大的残余缺陷尺寸。f-DBM（25）和 SCB（25）的骨愈合评分较高。纤维形式的 DBM 与 BMC 的联合应用显示出有前途的结果，可与金标准同种异体松质骨相媲美。纤维的更紧密堆积或更高量的磷酸钙没有积极的效果。
