Laboratoire Mécanique des Sols, Structures et Matériaux, UMR CNRS 8579, École Centrale Paris, Châtenay-Malabry Cedex, France.
Tissue Eng Part C Methods. 2011 May;17(5):505-16. doi: 10.1089/ten.TEC.2010.0468. Epub 2011 Feb 17.
A perfusion bioreactor, which was designed based on fluidized bed concepts, was validated for the culture of bone constructs of clinically relevant size. For this study, natural coral has been used as three-dimensional scaffolds. This biomaterial is a microporous, biocompatible, osteoconductive, and absorbable scaffold. This perfusion bioreactor provided a stable environment in terms of osmolarity, pH, and, most importantly, oxidative stress. Bone constructs engineered in this system resulted in significantly higher cell proliferation and homogenous cell distribution than those cultured under static conditions. Particularly relevant to the production of bioengineered bone in a clinical setting, custom-made bone constructs (each one with volume up to 30 cm(3)) could be produced using a such perfusion bioreactor. Last, but not least, the bone constructs of clinically relevant volume thus produced were shown to be osteogenic when transplanted subcutaneously in sheep.
一种基于流化床概念设计的灌注式生物反应器已被验证可用于培养具有临床相关尺寸的骨构建体。在这项研究中，天然珊瑚被用作三维支架。这种生物材料具有微孔、生物相容性、骨诱导性和可吸收性。这种灌注式生物反应器在渗透压、pH 值以及最重要的氧化应激方面提供了一个稳定的环境。与在静态条件下培养的细胞相比，在该系统中构建的骨组织具有更高的细胞增殖率和更均匀的细胞分布。特别值得一提的是，在临床环境中生产生物工程骨时，使用这种灌注式生物反应器可以生产出定制的骨构建体（每个构建体的体积可达 30 立方厘米）。最后但同样重要的是，当这些具有临床相关体积的骨构建体被移植到绵羊的皮下时，表现出了成骨特性。
