Sadri Hassan, Larki Negar Nowroozi, Kolahian Saeed
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, 516616471, Iran.
Department of Pharmacology and Experimental Therapy, Institute of Experimental and Clinical Pharmacology and Toxicology and ICePhA, University of Tuebingen, 72074, Tuebingen, Germany.
Biol Trace Elem Res. 2017 Dec;180(2):246-254. doi: 10.1007/s12011-017-1014-2. Epub 2017 Apr 13.
For the increasing development of diabetes, dietary habits and using appropriate supplements can play important roles in the treatment or reduction of risk for this disease. The objective of this study was to investigate the effects of leucine (Leu), zinc (Zn), and chromium (Cr) supplementation, alone or in combination, in rats with type 2 diabetes (T2D). Seventy-seven adult male Wistar rats were randomly assigned in 11 groups, using nutritional supplements and insulin (INS) or glibenclamide (GLC). Supplementing Leu significantly reduced blood glucose, triglycerides (TG), nonesterified fatty acids (NEFA), low-density lipoprotein (LDL), and increased high-density lipoprotein (HDL) concentrations compared to vehicle-treated T2D animals, and those improvements were associated with reduced area under the 2-h blood glucose response curve (AUC). Supplementation of T2D animals with Zn improved serum lipid profile as well as blood glucose concentrations but was not comparable with the INS, GLC, and Leu groups. Supplementary Cr did not improve blood glucose and AUC in T2D rats, whereas it reduced serum TG and LDL and increased HDL concentrations. In conclusion, supplementation of diabetic rats with Leu was more effective in improving blood glucose and consequently decreasing glucose AUC than other nutritional supplements. Supplementary Zn and Cr only improved serum lipid profile. The combination of the nutritional supplements did not improve blood glucose level. Nevertheless, supplementation with Leu-Zn, Leu-Cr, Zn-Cr, and Leu-Zn-Cr led to an improved response in serum lipid profile over each supplement given alone.
随着糖尿病病情的不断发展，饮食习惯和使用适当的补充剂在该疾病的治疗或风险降低方面可发挥重要作用。本研究的目的是调查单独或联合补充亮氨酸（Leu）、锌（Zn）和铬（Cr）对2型糖尿病（T2D）大鼠的影响。77只成年雄性Wistar大鼠被随机分为11组，使用营养补充剂以及胰岛素（INS）或格列本脲（GLC）。与接受载体处理的T2D动物相比，补充Leu可显著降低血糖、甘油三酯（TG）、非酯化脂肪酸（NEFA）、低密度脂蛋白（LDL），并提高高密度脂蛋白（HDL）浓度，这些改善与2小时血糖反应曲线下面积（AUC）的降低有关。给T2D动物补充Zn可改善血脂谱以及血糖浓度，但与INS、GLC和Leu组相比效果不佳。补充Cr并未改善T2D大鼠的血糖和AUC，然而它降低了血清TG和LDL，并提高了HDL浓度。总之，与其他营养补充剂相比，给糖尿病大鼠补充Leu在改善血糖并因此降低血糖AUC方面更有效。补充Zn和Cr仅改善了血脂谱。营养补充剂的组合并未改善血糖水平。尽管如此，补充Leu-Zn、Leu-Cr、Zn-Cr和Leu-Zn-Cr导致血清脂质谱的反应比单独给予每种补充剂时有所改善。
