Vasconcelos Ana M, Daam Michiel A, de Resende Juliana C, Casali-Pereira Maressa P, Espíndola Evaldo L G
NEEA/CRHEA/SHS, São Carlos Engineering School, University of São Paulo, Av. Trabalhador São Carlense, 400, 13.560-970, São Carlos, Brazil.
CENSE, Department of Environmental Sciences and Engineering, Faculty of Sciences and Technology, New University of Lisbon, Quinta da Torre, Caparica, 2829-516, Portugal.
Ecotoxicology. 2017 Aug;26(6):729-737. doi: 10.1007/s10646-017-1804-5. Epub 2017 Apr 13.
As compared to other aquatic taxonomic groups, few studies have been conducted so far evaluating the potential risks of pesticides to amphibians. Furthermore, most existing studies with amphibians consist of acute laboratory toxicity tests that mostly only evaluated single peak pesticide exposure. In the present study, potential chronic effects of single and repeated abamectin applications on the survival and development of L. catesbeianus tadpoles under (semi-) field conditions were evaluated. To this end, tadpoles were housed in microcosms treated with single or repeated applications of abamectin (as the commercial product Vertimec® 18 EC). The single application level corresponded to the previously established laboratory 96 h LC50 of the test organism, whereas the repeated application was based on abamectin concentrations likely to occur in runoff water from agricultural areas where Vertimec® 18 EC is applied. Under semi-field conditions, toxicity after the single application was greater than would be expected from the laboratory toxicity value. Although the repeated application did not exert direct effects on tadpole survival, the observed delay in development may have pronounced effect on the fitness and survival of anuran populations in edge-of-field water bodies.
与其他水生生物分类群相比，到目前为止，评估农药对两栖动物潜在风险的研究较少。此外，大多数现有的关于两栖动物的研究包括急性实验室毒性试验，这些试验大多只评估了单次峰值农药暴露情况。在本研究中，评估了在（半）田间条件下单次和重复施用阿维菌素对牛蛙蝌蚪存活和发育的潜在慢性影响。为此，将蝌蚪饲养在用单次或重复施用阿维菌素（作为商业产品敌杀死®18 EC）处理过的微型生态系统中。单次施用水平对应于先前确定的受试生物的实验室96小时半数致死浓度，而重复施用则基于可能出现在施用敌杀死®18 EC的农业区域径流水中的阿维菌素浓度。在半田间条件下，单次施用后的毒性大于根据实验室毒性值预期的毒性。虽然重复施用对蝌蚪存活没有直接影响，但观察到的发育延迟可能对田间边缘水体中无尾两栖动物种群的适应性和存活产生显著影响。
