Laboratório de Pesquisas Biológicas, Instituto Federal Goiano-Campus Urutaí, Rodovia Geraldo Silva Nascimento, 2,5 km, Zona Rural, Urutaí, GO, 75790-000, Brazil.
Departamento de Ciências Biológicas, Programa de Pós-Graduação em Conservação de Recursos Naturais do Cerrado, Instituto Federal Goiano-Campus Urutaí, Urutaí, GO, Brazil.
Environ Sci Pollut Res Int. 2017 Oct;24(29):23411-23416. doi: 10.1007/s11356-017-0124-x. Epub 2017 Sep 11.
Abamectin is one of the most used pesticides worldwide. However, investigations about its effects on amphibian populations are rare. Thus, the present study sought to investigate possible cytotoxic effects on Lithobates catesbeianus tadpoles exposed to low abamectin concentrations diluted in water. Accordingly, four experimental groups were set: negative control, positive control (cyclophosphamide-40 mL L), abamectin at concentrations 36 μg a.i./L (ABA36 group), and 72 μg a.i./L (ABA72 group), applied as Kraft® 36EC. The micronucleus test was conducted and other nuclear abnormalities in peripheral blood erythrocytes were checked after 24, 48, and 72 h of exposure to the treatments. The total of other nuclear abnormalities was influenced by the treatments, whereas the frequency of micronuclei was influenced by the exposure time. Such frequency was higher in the animals comprising the ABA72 group, which was assessed 72 h after the exposure had begun. The total of other nuclear abnormalities was influenced by the treatments. Animals in the positive control, ABA36, and ABA72 groups showed similar frequency of these abnormalities at 48 and 72 h. However, this frequency was statistically higher than that of animals in their respective negative control groups. Thus, the present study confirmed the hypothesis that the exposure of L. catesbeianus tadpoles to abamectin caused cytotoxic effects on them, although this exposure lasted short and the concentrations were low. It disclosed prospects for variations in the nucleus of erythrocytes circulating in amphibians, a fact that may provide an important/complementary approach for the detection of cytotoxicity caused by abamectin exposure.
阿维菌素是世界上使用最广泛的农药之一。然而，关于其对两栖动物种群影响的调查很少。因此，本研究旨在调查低浓度阿维菌素在水中稀释后对牛蛙蝌蚪可能产生的细胞毒性作用。为此，设置了四个实验组：阴性对照组、阳性对照组（环磷酰胺 40 mL L）、阿维菌素浓度为 36μg a.i./L（ABA36 组）和 72μg a.i./L（ABA72 组），均以 Kraft®36EC 形式应用。进行了微核试验，并在暴露于处理后 24、48 和 72 小时检查外周血红细胞中的其他核异常。其他核异常的总数受处理的影响，而微核的频率受暴露时间的影响。在暴露开始后 72 小时评估的 ABA72 组动物中，微核的频率更高。其他核异常的总数受处理的影响。阳性对照组、ABA36 组和 ABA72 组的动物在 48 和 72 小时时具有相似的异常频率。然而，这种频率明显高于各自阴性对照组的动物。因此，本研究证实了这样的假设，即阿维菌素暴露于牛蛙蝌蚪会对其产生细胞毒性作用，尽管暴露时间短且浓度低。它揭示了在两栖动物中循环的红细胞核发生变化的前景，这一事实可能为检测阿维菌素暴露引起的细胞毒性提供了重要/补充方法。
