Kwon W-S, Rahman M S, Ryu D-Y, Khatun A, Pang M-G
Department of Animal Science & Technology, Chung-Ang University, Anseong, Gyeonggi-Do, Korea.
Andrology. 2017 May;5(3):568-577. doi: 10.1111/andr.12332.
To overcome the limitations of conventional analysis of male fertility in animals and humans, proteomic studies have been performed to develop fertility-related biomarkers for prognosis and diagnosis of male fertility. However, the studies were focused on specific species or breeds. Therefore, a study is required to validate whether fertility-related markers would apply to other breeds in pigs. In this study, previously developed fertility-related biomarkers from Landrace were validated to use for prognosis of male fertility in commercially available breeds. Expression level of eight biomarkers in non-capacitated and capacitated (C) spermatozoa from Yorkshire and Duroc boars was analyzed. And then, to explore the validity of these markers for prognosis of male fertility, i.e. litter size, artificial insemination was performed. Among them, RAB2A (NC) and UQCRC1 (NC) turned out to be highest efficient markers for Yorkshire. RAB2A (C) was most efficient marker for Duroc. Average litter size has increased as much as 1.41 live born after prediction using eight fertility-related biomarkers in Yorkshire. In addition, average 2.52 litter size was increased after prediction using eight fertility-related biomarkers in Duroc. Average litter sizes were especially highly increased after prediction of fertility using RAB2A (NC) in Yorkshire (1.57 piglets) and TPI (NC) in Duroc (3.14 piglets), respectively. As a result, all biomarkers were significantly correlated with litter size. However, overall accuracy to predict litter size in three breeds was different in response with each marker. Average litter size after artificial insemination was also significantly affected by marker selection. Therefore, this study suggests that developed fertility-related markers may be used for prognosis and diagnosis of male fertility irrespective of breed. However, selection of efficient markers for breeds should be considered to obtain more accurate and efficient outcomes.
为克服动物和人类男性生育力传统分析方法的局限性，已开展蛋白质组学研究以开发与生育力相关的生物标志物，用于男性生育力的预后和诊断。然而，这些研究集中在特定物种或品种上。因此，需要一项研究来验证与生育力相关的标志物是否适用于猪的其他品种。在本研究中，对先前从长白猪中开发的与生育力相关的生物标志物进行了验证，以用于市售品种公猪的男性生育力预后评估。分析了约克夏猪和杜洛克猪未获能和获能（C）精子中8种生物标志物的表达水平。然后，为探究这些标志物对男性生育力（即产仔数）预后评估的有效性，进行了人工授精。其中，RAB2A（未获能）和UQCRC1（未获能）被证明是约克夏猪最有效的标志物。RAB2A（获能）是杜洛克猪最有效的标志物。在约克夏猪中，使用8种与生育力相关的生物标志物进行预测后，平均产仔数增加了多达1.41头活产仔猪。此外，在杜洛克猪中，使用8种与生育力相关的生物标志物进行预测后，平均产仔数增加了2.52头。分别使用RAB2A（未获能）对约克夏猪（1.57头仔猪）和TPI（未获能）对杜洛克猪（3.14头仔猪）进行生育力预测后，平均产仔数尤其显著增加。结果，所有生物标志物均与产仔数显著相关。然而，三种品种中预测产仔数的总体准确性因每个标志物而异。人工授精后的平均产仔数也受到标志物选择的显著影响。因此，本研究表明，所开发的与生育力相关的标志物可用于男性生育力的预后和诊断，而不论品种如何。然而，为获得更准确和有效的结果，应考虑为不同品种选择有效的标志物。
