Omar Y A, Younis S E, Ismail I Y, El-Sakka A I
Department of Urology, Suez Canal University, Ismailia, Egypt.
Department of Clinical Pathology, Suez Canal University, Ismailia, Egypt.
Andrology. 2017 May;5(3):527-534. doi: 10.1111/andr.12347.
The association between endothelial dysfunction and late onset hypogonadism (LOH) in patients with vasculogenic erectile dysfunction (ED) is not yet well settled. Our objective was to assess the association between LOH and endothelial dysfunction in patients with vasculogenic ED. Throughout 2014-2015 a total of 90 men were enrolled in this cross-sectional observational study. Of them 60 patients with a clinical diagnosis of ED were further subdivided into two equal groups: patients with vasculogenic ED and LOH (A); patients with vasculogenic ED and euogonadal (B). Thirty age-matched men with no ED or hypogonadism were enrolled as control group (C). All patients were subjected to detailed medical and sexual history, total testosterone (TT), calculated free (FT) and bioavailable testosterone (BT), flow cytometric evaluation for endothelial progenitor cells (EPCs) (CD45negative/CD34positive/CD144positive) and endothelial microparticles (EMPs) (CD45negative/CD144positive/annexin V positive). The mean age ± SD of the three groups A, B and C were 51.3 ± 11.1, 53.6 ± 10.6 and 48.3 ± 5 years, respectively, with insignificant age differences (p = 0.089). The diagnostic criteria of LOH were adapted according to European male aging study, 2010. The means of TT(ng/mL) were 2.32 ± 0.21, 6.43 ± 0.36 and 5.37 ± 0.30 in groups A, B and C, respectively. There were highly significant differences between group A and groups B and C (p < 0.001 for each). The means of EPCs were 0.43 ± 0.070, 0.22 ± 0.05 and 0.032 ± 0.013 in groups A, B and C, respectively. The means of EMPs were 0.15 ± 0.029, 0.056 ± .013 and 0.014 ± 0.002 in groups A, B and C, respectively. There were significant differences between group C and groups A and B (p < 0.05 for each). This study clearly demonstrated that there is a significant association between LOH and the higher expression of EPCs and EMPs in patients with vasculogenic ED.
血管性勃起功能障碍（ED）患者中，内皮功能障碍与迟发性性腺功能减退（LOH）之间的关联尚未完全明确。我们的目的是评估血管性ED患者中LOH与内皮功能障碍之间的关联。在2014年至2015年期间，共有90名男性参与了这项横断面观察性研究。其中，60例临床诊断为ED的患者被进一步分为两组：血管性ED合并LOH的患者（A组）；血管性ED合并性腺功能正常的患者（B组）。另外招募了30名年龄匹配、无ED或性腺功能减退的男性作为对照组（C组）。所有患者均接受了详细的病史和性史询问、总睾酮（TT）、计算游离睾酮（FT）和生物可利用睾酮（BT）检测、内皮祖细胞（EPCs）（CD45阴性/CD34阳性/CD144阳性）和内皮微粒（EMPs）（CD45阴性/CD144阳性/膜联蛋白V阳性）的流式细胞术评估。A、B、C三组的平均年龄±标准差分别为51.3±11.1岁、53.6±10.6岁和48.3±5岁，年龄差异无统计学意义（p = 0.089）。LOH的诊断标准参照2010年欧洲男性衰老研究。A、B、C三组的TT均值（ng/mL）分别为2.32±0.21、6.43±0.36和5.37±0.30。A组与B组和C组之间存在高度显著差异（每组p < 0.001）。A、B、C三组的EPCs均值分别为0.43±0.070、0.22±0.05和0.032±0.013。A、B、C三组的EMPs均值分别为0.15±0.029、0.056±0.013和0.014±0.002。C组与A组和B组之间存在显著差异（每组p < 0.05）。本研究清楚地表明，血管性ED患者中，LOH与EPCs和EMPs的高表达之间存在显著关联。
