Fang Xin, Xu Xi-Ke, Wang Guo-Wei, Zeng Ren-Tao, Tian Xin-Hui, Shi Zhi-Ran, Zhuo Zhi-Guo, Shen Yun-Heng, Zhang Wei-Dong
Department of Phytochemistry, Second Military Medical University, Shanghai 200433, People's Republic of China.
Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, People's Republic of China.
Phytochemistry. 2017 Jul;139:47-55. doi: 10.1016/j.phytochem.2017.04.001. Epub 2017 Apr 12.
Six previously undescribed C-guaianolides, a previously undescribed guaianolide alkaloid, and two previously undescribed guaianolides as well as 10 known guaianolides were obtained from an ethanol extract of Ainsliaea yunnanensis Franch. The chemical structures of all previously reported sesquiterpenoids were determined by extensive NMR spectroscopic analysis in combination with a modified Mosher's method. All isolates were in vitro screened for inhibitory effect against nitric oxide release in RAW 264.7 macrophages stimulated by LPS. Zaluzanin C remarkably inhibited the production of nitric oxide with an IC value of 6.54 μM.
从云南兔儿风（Ainsliaea yunnanensis Franch.）的乙醇提取物中获得了6个此前未被描述的愈创木烷型倍半萜内酯、1个此前未被描述的愈创木烷型倍半萜生物碱、2个此前未被描述的愈创木烷型倍半萜内酯以及10个已知的愈创木烷型倍半萜内酯。所有此前报道的倍半萜类化合物的化学结构均通过广泛的核磁共振光谱分析结合改良的莫舍尔方法确定。对所有分离物进行体外筛选，以检测其对脂多糖刺激的RAW 264.7巨噬细胞中一氧化氮释放的抑制作用。扎鲁扎宁C显著抑制一氧化氮的产生，IC值为6.54 μM。
