Schott D R, East P D, Paigen K
Department of Genetics, University of California, Berkeley 94720.
Genetics. 1988 Jul;119(3):631-7. doi: 10.1093/genetics/119.3.631.
We describe the characterization of a previously reported control mutation, AdhSL, in the alcohol dehydrogenase gene of Drosophila melanogaster, which results in decreased production of ADH molecules and subsequently lower ADH activity in adults. We find that the regulatory element modifies ADH mRNA levels and acts cis on both ADH protein and mRNA. It is not promoter specific but is developmentally specific to the adult stage. The AdhSL allele carries a 4.5-kb insert approximately 3 kb 5' to the distal promoter. This new insertion may be responsible for the regulatory phenotype of AdhSL.
我们描述了果蝇酒精脱氢酶基因中一个先前报道的调控突变AdhSL的特征，该突变导致成年果蝇体内ADH分子产量降低，进而使ADH活性降低。我们发现该调控元件可改变ADH mRNA水平，并且对ADH蛋白和mRNA均起顺式作用。它不是启动子特异性的，而是在发育上对成虫阶段具有特异性。AdhSL等位基因在距远端启动子约3 kb的5'端携带一个4.5 kb的插入片段。这个新的插入片段可能是AdhSL调控表型的原因。
