黑腹果蝇中AdhSL调控突变的特征分析。
Characterization of the AdhSL regulatory mutation in Drosophila melanogaster.
作者信息
Schott D R, East P D, Paigen K
机构信息
Department of Genetics, University of California, Berkeley 94720.
出版信息
Genetics. 1988 Jul;119(3):631-7. doi: 10.1093/genetics/119.3.631.
Abstract
We describe the characterization of a previously reported control mutation, AdhSL, in the alcohol dehydrogenase gene of Drosophila melanogaster, which results in decreased production of ADH molecules and subsequently lower ADH activity in adults. We find that the regulatory element modifies ADH mRNA levels and acts cis on both ADH protein and mRNA. It is not promoter specific but is developmentally specific to the adult stage. The AdhSL allele carries a 4.5-kb insert approximately 3 kb 5' to the distal promoter. This new insertion may be responsible for the regulatory phenotype of AdhSL.
摘要
我们描述了果蝇酒精脱氢酶基因中一个先前报道的调控突变AdhSL的特征，该突变导致成年果蝇体内ADH分子产量降低，进而使ADH活性降低。我们发现该调控元件可改变ADH mRNA水平，并且对ADH蛋白和mRNA均起顺式作用。它不是启动子特异性的，而是在发育上对成虫阶段具有特异性。AdhSL等位基因在距远端启动子约3 kb的5'端携带一个4.5 kb的插入片段。这个新的插入片段可能是AdhSL调控表型的原因。
相似文献
1
Characterization of the AdhSL regulatory mutation in Drosophila melanogaster.黑腹果蝇中AdhSL调控突变的特征分析。
Genetics. 1988 Jul;119(3):631-7. doi: 10.1093/genetics/119.3.631.
2
Identification of cis-regulatory elements required for larval expression of the Drosophila melanogaster alcohol dehydrogenase gene.黑腹果蝇乙醇脱氢酶基因幼虫表达所需顺式调控元件的鉴定
Genetics. 1990 Mar;124(3):637-46. doi: 10.1093/genetics/124.3.637.
3
Conserved enhancer and silencer elements responsible for differential Adh transcription in Drosophila cell lines.负责果蝇细胞系中乙醇脱氢酶（Adh）差异转录的保守增强子和沉默子元件。
Mol Cell Biol. 1990 Jul;10(7):3512-23. doi: 10.1128/mcb.10.7.3512-3523.1990.
4
Isolation of silencer-containing sequences causing a tissue-specific position effect on alcohol dehydrogenase expression in Drosophila melanogaster.在黑腹果蝇中，分离出对乙醇脱氢酶表达产生组织特异性位置效应的含沉默子序列。
Dev Genet. 1994;15(2):188-200. doi: 10.1002/dvg.1020150209.
5
Regulatory elements involved in Drosophila Adh gene expression are conserved in divergent species and separate elements mediate expression in different tissues.果蝇乙醇脱氢酶（Adh）基因表达所涉及的调控元件在不同物种中是保守的，且不同元件介导在不同组织中的表达。
EMBO J. 1986 Jun;5(6):1275-89. doi: 10.1002/j.1460-2075.1986.tb04357.x.
6
Insertion of the retroposable element, jockey, near the Adh gene of Drosophila melanogaster is associated with altered gene expression.
Genet Res. 1996 Dec;68(3):203-9. doi: 10.1017/s0016672300034170.
7
cis-Acting sequences controlling the adult-specific transcription pattern of the Drosophila affinidisjuncta Adh gene.控制果蝇affinidisjuncta Adh基因成虫特异性转录模式的顺式作用序列。
Dev Genet. 1998;23(2):119-27. doi: 10.1002/(SICI)1520-6408(1998)23:2<119::AID-DVG4>3.0.CO;2-9.
8
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8. doi: 10.1073/pnas.77.10.5794.
9
A KP element inserted between the two promoters of the alcohol dehydrogenase gene of Drosophila melanogaster differentially affects expression in larvae and adults.一个插入在黑腹果蝇乙醇脱氢酶基因的两个启动子之间的KP元件对幼虫和成虫中的表达有不同影响。
Biochem Genet. 1998 Dec;36(11-12):363-79. doi: 10.1023/a:1018749513875.
10
The Adh-related gene of Drosophila melanogaster is expressed as a functional dicistronic messenger RNA: multigenic transcription in higher organisms.果蝇的乙醇脱氢酶相关基因表达为一种功能性双顺反子信使核糖核酸：高等生物中的多基因转录。
EMBO J. 1997 Apr 15;16(8):2023-31. doi: 10.1093/emboj/16.8.2023.
引用本文的文献
1
Species-specific differences in tissue-specific expression of alcohol dehydrogenase are under the control of complex cis-acting loci: evidence from Drosophila hybrids.酒精脱氢酶组织特异性表达中的物种特异性差异受复杂顺式作用位点的控制：来自果蝇杂交种的证据。
Biochem Genet. 1991 Dec;29(11-12):577-92. doi: 10.1007/BF02426872.
本文引用的文献
1
Gene transformation.基因转化
Am Sci. 1982 Sep-Oct;70(5):496-505.
2
Analysis of genetic variation affecting the relative activities of fast and slow ADH dimers in Drosophila melanogaster heterozygotes.影响黑腹果蝇杂合子中快速和慢速乙醇脱氢酶二聚体相对活性的遗传变异分析。
Biochem Genet. 1980 Aug;18(7-8):765-80. doi: 10.1007/BF00484592.
3
Alcohol dehydrogenase in Drosophila: isolation and characterization of messenger RNA and cDNA clone.果蝇中的乙醇脱氢酶：信使核糖核酸和互补脱氧核糖核酸克隆的分离与特性分析
Nucleic Acids Res. 1980 Dec 11;8(23):5649-67. doi: 10.1093/nar/8.23.5649.
4
Isolation and partial characterization of the Drosophila alcohol dehydrogenase gene.果蝇乙醇脱氢酶基因的分离与部分特性分析
Proc Natl Acad Sci U S A. 1980 Oct;77(10):5794-8. doi: 10.1073/pnas.77.10.5794.
5
The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.黑腹果蝇中乙醇脱氢酶的信使核糖核酸在不同发育阶段，其5'端有所不同。
Cell. 1983 May;33(1):125-33. doi: 10.1016/0092-8674(83)90341-0.
6
Genetic control of Adh expression in Drosophila melanogaster.黑腹果蝇中乙醇脱氢酶（Adh）表达的遗传控制。
Genetics. 1983 Dec;105(4):921-33. doi: 10.1093/genetics/105.4.921.
7
A family of oligo-adenylate-terminated transposable sequences in Drosophila melanogaster.黑腹果蝇中一类以寡聚腺苷酸结尾的转座序列家族。
J Mol Biol. 1983 Aug 25;168(4):715-27. doi: 10.1016/s0022-2836(83)80071-0.
8
A transposable element inserted just 5' to a Drosophila glue protein gene alters gene expression and chromatin structure.一个转座元件插入到果蝇黏蛋白基因的5'端上游，会改变基因表达和染色质结构。
Cell. 1983 Aug;34(1):75-84. doi: 10.1016/0092-8674(83)90137-x.
9
Structures of P transposable elements and their sites of insertion and excision in the Drosophila melanogaster genome.果蝇基因组中P转座因子的结构及其插入和切除位点。
Cell. 1983 Aug;34(1):25-35. doi: 10.1016/0092-8674(83)90133-2.
10
A transposable element that splits the promoter region inactivates a Drosophila cuticle protein gene.一个可转座元件分裂启动子区域，使果蝇表皮蛋白基因失活。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7430-4. doi: 10.1073/pnas.79.23.7430.
Zotero 插件