Benyajati C, Spoerel N, Haymerle H, Ashburner M
Cell. 1983 May;33(1):125-33. doi: 10.1016/0092-8674(83)90341-0.
Alcohol dehydrogenase (EC 1.1.1.1) of Drosophila melanogaster is coded by a single structural gene, active in both larvae and adults. The major larval and adult transcripts of Adh differ in their 5'-untranslated regions. The major larval mRNA is about 1100 bases long, some 50 bases shorter than the major adult transcript. The 5' end of the larval mRNA is colinear with the genomic sequence immediately adjacent to the coding region, starting 70 base pairs (bp) upstream of the initiation codon. By contrast, the adult mRNA shares only 36 of its 123 5'-untranslated bases with the larval mRNA; the remaining 87 are encoded by a sequence 654 bp upstream. Both initiation sites are preceded by a TATA box some 24 bp upstream. The developmental specificity of Adh expression is seen, therefore, to have a counterpart in the specificity of transcription initiation at the two separate promoter regions.
黑腹果蝇的乙醇脱氢酶（EC 1.1.1.1）由单个结构基因编码，在幼虫和成虫中均有活性。Adh的主要幼虫和成虫转录本在其5'-非翻译区有所不同。主要的幼虫mRNA约1100个碱基长，比主要的成虫转录本短约50个碱基。幼虫mRNA的5'端与紧邻编码区的基因组序列共线，起始于起始密码子上游70个碱基对（bp）处。相比之下，成虫mRNA的123个5'-非翻译碱基中只有36个与幼虫mRNA相同；其余87个由上游654 bp处的序列编码。两个起始位点上游约24 bp处均有一个TATA框。因此，Adh表达的发育特异性在两个独立启动子区域转录起始的特异性中也有体现。
