da Silva Almeida Ana Paula, Avi Camilla Martins, Barbisan Luís Fernando, de Moura Nelci Antunes, Caetano Brunno Felipe Ramos, Romualdo Guilherme Ribeiro, Sivieri Kátia
Department of Food and Nutrition, Faculty of Pharmaceutical Science, UNESP - UnivEstadual Paulista Araraquara, SP, Brazil.
Department of Morphology, Institute of Biosciences, UNESP-São Paulo State University, Botucatu, SP, Brazil.
Food Res Int. 2015 Aug;74:48-54. doi: 10.1016/j.foodres.2015.04.034. Epub 2015 Apr 24.
The modifying effects of aqueous yacon extract (AYE) and Lactobacillus acidophilus CRL 1014 against colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats were investigated. Animals were allocated into five groups: G1: untreated group; G2: DMH-treated group; G3: DMH+L. acidophilus-treated group; G4: DMH+AYE-treated group; G5: DMH+L. acidophilus and AYE-treated group. A significant reduction (p<0.05) in leukocyte DNA damage and in colonic cell proliferation was observed after the first DMH administration in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. In this moment, a significant increase (p<0.05) in colonic apoptosis was also observed in G3 (probiotic) and G5 (synbiotic) groups. In special, at five months after DMH administrations, a significant reduction (p<0.05) in ACF development was observed in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. Incidence of colon tumors was lower at five months in G4 (prebiotic) and G5 (synbiotic) groups but not in eight months after DMH administrations. In conclusion, the findings suggest that the oral treatments have potential effects as a chemopreventive agent against colon carcinogenesis on an early stage of tumor development.
研究了雪莲果水提取物（AYE）和嗜酸乳杆菌CRL 1014对雄性Wistar大鼠1,2-二甲基肼（DMH）诱导的结肠癌发生的调节作用。将动物分为五组：G1：未处理组；G2：DMH处理组；G3：DMH+嗜酸乳杆菌处理组；G4：DMH+AYE处理组；G5：DMH+嗜酸乳杆菌和AYE处理组。在G3（益生菌）、G4（益生元）和G5（合生元）组首次给予DMH后，观察到白细胞DNA损伤和结肠细胞增殖显著降低（p<0.05）。此时，在G3（益生菌）和G5（合生元）组中也观察到结肠细胞凋亡显著增加（p<0.05）。特别地，在给予DMH五个月后，在G3（益生菌）、G4（益生元）和G5（合生元）组中观察到ACF发育显著降低（p<0.05）。在给予DMH五个月时，G4（益生元）和G5（合生元）组的结肠肿瘤发生率较低，但在给予DMH八个月后则不然。总之，研究结果表明，口服治疗在肿瘤发生发展的早期阶段作为结肠癌化学预防剂具有潜在作用。
