Zadravec Marko, Slavec Brigita, Krapež Uroš, Gombač Mitja, Švara Tanja, Poljšak-Prijatelj Mateja, Gruntar Igor, Račnik Joško
Veterinary Faculty, Institute of Poultry, Birds, Small Mammals and Reptiles, Gerbičeva 60, 1000 Ljubljana, Slovenia.
Veterinary Faculty, Institute of Pathology, Wildlife, Fish and Bees, Gerbičeva 60, 1000 Ljubljana, Slovenia.
Vet Parasitol. 2017 May 30;239:90-93. doi: 10.1016/j.vetpar.2017.04.001. Epub 2017 Apr 3.
In the present paper, an outbreak of trichomonosis in a flock of 15 breeding pairs of canaries is described. Trichomonosis was diagnosed on characteristic clinical signs, microscopic examination of crop/esophageal swabs, gross pathology and histopathology. Trichomonads were successfully grown in culture media and were characterized by multi-locus sequence typing. The three genomic loci ITS1-5.8S-ITS2, 18S rRNA and Fe-hydrogenase were analyzed. Molecular characterization confirmed the finch trichomonosis strain, identical to the strain that caused emerging disease in free-living passerine birds in Europe. Flock treatment with metronidazole (200mg/L) in drinking water for 5days was partially effective. After individual treatment with oral application of metronidazole (20mg/kg SID) for 5days no further clinical signs were observed in the flock over next 30 months.
在本文中,描述了一群15对繁殖用金丝雀爆发毛滴虫病的情况。通过特征性临床症状、嗉囊/食管拭子的显微镜检查、大体病理学和组织病理学诊断出毛滴虫病。毛滴虫在培养基中成功培养,并通过多位点序列分型进行特征分析。分析了三个基因组位点ITS1-5.8S-ITS2、18S rRNA和铁氢化酶。分子特征鉴定证实该雀科毛滴虫菌株与在欧洲自由生活的雀形目鸟类中引发新出现疾病的菌株相同。在饮用水中添加甲硝唑(200mg/L)对鸟群进行为期5天的治疗有部分效果。在对个体口服甲硝唑(20mg/kg,每日一次)进行为期5天的治疗后,在接下来的30个月里,鸟群中未观察到进一步的临床症状。