Leider J M, Palese P, Smith F I
Department of Microbiology, Mount Sinai School of Medicine, New York, New York 10029.
J Virol. 1988 Sep;62(9):3084-91. doi: 10.1128/JVI.62.9.3084-3091.1988.
The mutation rate of Rous sarcoma virus (RSV) was measured. Progeny descended from a single virion were collected after one replication cycle, and seven regions of the genome were analyzed for mutations by denaturing-gradient gel electrophoresis. In all, 65,250 nucleotides were screened, yielding nine mutations, and the RSV mutation rate was calculated as 1.4 x 10(-4) mutations per nucleotide per replication cycle. These results indicate that RSV is an extremely mutable virus. We speculate that the mutation rate of a virus may correlate inversely with the effectiveness of vaccination against a given virus and suggest that prevention of retrovirus-mediated disease via vaccination may prove difficult.
对劳氏肉瘤病毒（RSV）的突变率进行了测定。在一个复制周期后收集源自单个病毒粒子的子代病毒，并通过变性梯度凝胶电泳分析基因组的七个区域的突变情况。总共筛选了65250个核苷酸，产生了9个突变，RSV的突变率计算为每个复制周期每核苷酸1.4×10⁻⁴个突变。这些结果表明RSV是一种极易突变的病毒。我们推测病毒的突变率可能与针对特定病毒的疫苗接种效果呈负相关，并表明通过疫苗接种预防逆转录病毒介导的疾病可能很困难。
