Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University and Guangdong Provincial Key Laboratory of Stomatology , Guangzhou 510055, Guangdong, People's Republic of China.
Institute of Health and Biomedical Innovation & the Australia-China Centre for Tissue Engineering and Regenerative Medicine, Queensland University of Technology , Brisbane, Queensland 4059, Australia.
ACS Nano. 2017 May 23;11(5):4494-4506. doi: 10.1021/acsnano.6b07808. Epub 2017 Apr 21.
Osteoimmunomodulation has informed the importance of modulating a favorable osteoimmune environment for successful materials-mediated bone regeneration. Nanotopography is regarded as a valuable strategy for developing advanced bone materials, due to its positive effects on enhancing osteogenic differentiation. In addition to this direct effect on osteoblastic lineage cells, nanotopography also plays a vital role in regulating immune responses, which makes it possible to utilize its immunomodulatory properties to create a favorable osteoimmune environment. Therefore, the aim of this study was to advance the applications of nanotopography with respect to its osteoimmunomodulatory properties, aiming to shed further light on this field. We found that tuning the surface chemistry (amine or acrylic acid) and scale of the nanotopography (16, 38, and 68 nm) significantly modulated the osteoimmune environment, including changes in the expression of inflammatory cytokines, osteoclastic activities, and osteogenic, angiogenic, and fibrogenic factors. The generated osteoimmune environment significantly affected the osteogenic differentiation of bone marrow stromal cells, with carboxyl acid-tailored 68 nm surface nanotopography offering the most promising outcome. This study demonstrated that the osteoimmunomodulation could be manipulated via tuning the chemistry and nanotopography, which implied a valuable strategy to apply a "nanoengineered surface" for the development of advanced bone biomaterials with favorable osteoimmunomodulatory properties.
骨免疫调节使人们认识到调节有利于骨再生的骨免疫环境的重要性。由于纳米形貌对增强成骨分化具有积极影响，因此被认为是开发先进骨材料的一种有价值的策略。除了对成骨细胞谱系细胞有直接影响外，纳米形貌在调节免疫反应方面也起着至关重要的作用，这使得利用其免疫调节特性来创造有利的骨免疫环境成为可能。因此，本研究旨在推进纳米形貌在骨免疫调节特性方面的应用，以期进一步阐明该领域。我们发现，调整表面化学（胺或丙烯酸）和纳米形貌的规模（16、38 和 68nm）可显著调节骨免疫环境，包括炎症细胞因子表达、破骨细胞活性以及成骨、血管生成和纤维生成因子的变化。所产生的骨免疫环境对骨髓基质细胞的成骨分化有显著影响，其中羧基酸修饰的 68nm 表面纳米形貌效果最显著。本研究表明，通过调整化学和纳米形貌可以实现骨免疫调节，这暗示了一种有价值的策略，即应用“纳米工程表面”来开发具有有利骨免疫调节特性的先进骨生物材料。
