Irani Mohammad, Sadeghi Gity Mir Mohamad, Haririan Ismaeil
Department of Chemical Engineering, Amirkabir University of Technology, Tehran, Iran.
Department of Polymer Engineering and Color Technology, Amirkabir University of Technology, P.O. Box 15875/4413, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:165-174. doi: 10.1016/j.msec.2017.02.029. Epub 2017 Feb 14.
In the present study, the PCL-Diol-b-PU/Au nanocompsite nanofibers were fabricated via electrospinning process during two different stages to load an anticancer temozolomide (TMZ) drug into the nanofibers. The first stage was the incorporation of Au nanoparticles into the nanofibers and the second stage was coating the gold nanoparticles on the surface of PCL-Diol-b-PU/Au composite nanofibers. The prepared nanofibrous formulations were characterized using FTIR, SEM and TEM analysis. Box-Behnken-design was used to investigate the influence of electrospinning parameters including solution concentration, applied voltage to tip-collector distance ratio and collector speed on the morphology and fiber diameter of PCL-Diol-b-PU/Au nanofibers. Drug loading efficiency, in vitro release profiles of TMZ from PCL-Diol-b-PU/Au and gold-coated PCL-Diol-b-PU/Au composite nanofibers as well as in vitro antitumor efficacy against U-87 MG human glioblastoma cells were carried out. The TMZ release data were well described using Korsmayer-Peppas kinetic model in which results indicated Fickian diffusion of TMZ from nanofibers. The obtained results revealed the higher efficiency of PCL-Diol-b-PU/Au@TMZ nanofibrous implants for treatment of glioblastoma tumors.
在本研究中，通过静电纺丝工艺在两个不同阶段制备了聚己内酯-二醇-嵌段-聚氨酯/金纳米复合纳米纤维，以便将抗癌药物替莫唑胺（TMZ）负载到纳米纤维中。第一阶段是将金纳米颗粒掺入纳米纤维中，第二阶段是在聚己内酯-二醇-嵌段-聚氨酯/金复合纳米纤维表面包覆金纳米颗粒。使用傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和透射电子显微镜（TEM）分析对制备的纳米纤维制剂进行表征。采用Box-Behnken设计研究静电纺丝参数（包括溶液浓度、施加电压与针尖-收集器距离之比以及收集器速度）对聚己内酯-二醇-嵌段-聚氨酯/金纳米纤维的形态和纤维直径的影响。进行了药物负载效率、替莫唑胺从聚己内酯-二醇-嵌段-聚氨酯/金和金包覆的聚己内酯-二醇-嵌段-聚氨酯/金复合纳米纤维的体外释放曲线以及对U-87 MG人胶质母细胞瘤细胞的体外抗肿瘤功效研究。替莫唑胺释放数据用Korsmayer-Peppas动力学模型进行了很好的描述，结果表明替莫唑胺从纳米纤维中以菲克扩散方式释放。所得结果表明聚己内酯-二醇-嵌段-聚氨酯/金@替莫唑胺纳米纤维植入物在治疗胶质母细胞瘤肿瘤方面具有更高的效率。
