Faculty of Pharmacy, Eastern Mediterranean University, Famagusta, North Cyprus via Mersin 10, Turkey.
Department of Chemistry, University of Sistan and Baluchestan, Zahedan, Iran.
Int J Biol Macromol. 2020 May 1;150:1130-1140. doi: 10.1016/j.ijbiomac.2019.10.120. Epub 2019 Nov 6.
The poly (ε-caprolactonediol) based polyurethane (PCL-Diol-b-PU)/poly(N-isopropylacrylamide)-grafted-chitosan (PNIPAAm-g-chitosan) core-shell nanofibers were synthesized via coaxial electrospinning process. Paclitaxel and 5-FU anticancer drugs were incorporated into the core of nanofibers. The nanofibers surface was coated using magnetic gold nanoparticles and the potential of synthesized nanofibers was investigated for the sustained release of paclitaxel and 5-FU toward 4T1 breast cancer cells death in vitro and in vivo. The synthesized magnetic nanoparticles were characterized using SEM, TEM, XRD and DLS analysis. The surface morphology of nanofibers was studied under various applied voltage and different shell flow rates. The paclitaxel and 5-FU release profiles from nanofibers were examined under acidic and physiological pH. The maximum 4T1 cell killing was found to be 78% using magnetic gold coated-nanofibers in the presence of external magnetic field. The SEM images after incubation of nanofibers in 4T1 breast cancer cells indicated the well adhesion of cells on the nanofibers surface. The in vivo studies showed that the tumor volume did not change during 10 days. The minimum increase in tumor volume was obtained using paclitaxel and 5-FU loaded-nanofibers coated by the magnetic gold nanoparticles. The obtained results demonstrated the high therapeutic efficiency of synthesized nanofibrous carrier toward breast cancer treatment.
聚（ε-己内酯二醇）基聚氨酯（PCL-Diol-b-PU）/接枝壳聚糖的聚（N-异丙基丙烯酰胺）（PNIPAAm-g-chitosan）核壳纳米纤维是通过同轴静电纺丝工艺合成的。紫杉醇和 5-FU 抗癌药物被包裹在纳米纤维的核心内。纳米纤维的表面用磁性金纳米粒子进行涂层，并研究了合成纳米纤维在体外和体内对 4T1 乳腺癌细胞死亡的紫杉醇和 5-FU 持续释放的潜力。使用 SEM、TEM、XRD 和 DLS 分析对合成的磁性纳米粒子进行了表征。研究了在不同施加电压和不同壳层流速下纳米纤维的表面形态。在酸性和生理 pH 条件下研究了纳米纤维中紫杉醇和 5-FU 的释放曲线。在存在外部磁场的情况下，使用磁性金涂层纳米纤维，发现对 4T1 细胞的最大杀伤率为 78%。将纳米纤维在 4T1 乳腺癌细胞中孵育后的 SEM 图像表明，细胞很好地附着在纳米纤维表面上。体内研究表明，在 10 天内肿瘤体积没有变化。用磁性金纳米粒子包裹的负载紫杉醇和 5-FU 的纳米纤维，肿瘤体积的最小增加。研究结果表明，合成的纤维状载体对乳腺癌治疗具有很高的治疗效率。
