Hadler Christoph, Wissel Kirsten, Brandes Gudrun, Dempwolf Wibke, Reuter Günter, Lenarz Thomas, Menzel Henning
Institute for Technical Chemistry, Braunschweig University of Technology, Germany.
Department of Otorhinolaryngology, Head and Neck Surgery, Hannover Medical School, Germany.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:286-296. doi: 10.1016/j.msec.2017.02.020. Epub 2017 Feb 10.
The polyimide Kapton® was coated photochemically with hydrophilic polymers to prevent undesirable cell growth on the polyimide surface. The polymer coatings were generated using photochemically reactive polymers synthesized by a simple and modular strategy. Suitable polymers or previously synthesized copolymer precursors were functionalized with photoactive arylazide groups by a polymer analogous amide coupling reaction with 4-azidobenzoic acid. A photoactive chitosan derivative (chitosan-Az) and photochemically reactive copolymers containing DMAA, DEAA or MTA as primary monomers were synthesized using this method. The amount of arylazide groups in the polymers was adjusted to approximately 5%, 10% and 20%. As coating on Kapton® all polymers effect a significantly reduced water contact angle (WCA) and consequently a rise of the surface hydrophilicity compared to the untreated Kapton®. The presence of the polymer coatings was also proven by ATR-IR spectroscopy. Coatings with chitosan-Az and the DEAA copolymer cause a distinct inhibition of the growth of fibroblasts. In the case of the DMAA copolymer even a strong anti-adhesive behavior towards fibroblasts was verified. Biocompatibility of the polymer coatings was proven which enables their utilization in biomedical applications.
聚酰亚胺Kapton®通过光化学方法涂覆亲水性聚合物，以防止聚酰亚胺表面出现不良细胞生长。聚合物涂层是使用通过简单且模块化策略合成的光化学反应性聚合物生成的。合适的聚合物或先前合成的共聚物前体通过与4-叠氮基苯甲酸的聚合物类似酰胺偶联反应，用光活性芳基叠氮基团进行功能化。使用该方法合成了光活性壳聚糖衍生物（壳聚糖-Az）以及以DMAA、DEAA或MTA作为主要单体的光化学反应性共聚物。聚合物中芳基叠氮基团的含量调整为约5%、10%和20%。作为Kapton®上的涂层，与未处理的Kapton®相比，所有聚合物均能显著降低水接触角（WCA），从而提高表面亲水性。聚合物涂层的存在也通过ATR-IR光谱得到证实。壳聚糖-Az涂层和DEAA共聚物涂层对成纤维细胞的生长有明显抑制作用。就DMAA共聚物而言，甚至证实其对成纤维细胞具有很强的抗粘附行为。已证明聚合物涂层具有生物相容性，这使其能够用于生物医学应用。
