Gokcekaya Ozkan, Ueda Kyosuke, Ogasawara Kouetsu, Kanetaka Hiroyasu, Narushima Takayuki
Department of Materials Processing, Tohoku University, 6-6-02 Aza Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Department of Materials Processing, Tohoku University, 6-6-02 Aza Aoba, Aramaki, Aoba-ku, Sendai, Miyagi 980-8579, Japan.
Mater Sci Eng C Mater Biol Appl. 2017 Jun 1;75:926-933. doi: 10.1016/j.msec.2017.02.059. Epub 2017 Feb 16.
Development of bioceramics with antibacterial activity and without cytotoxicity would be beneficial for preventing infection associated with implants. This study aimed to capitalize on the antibacterial properties of silver (Ag) incorporated in or coexisting in metallic form with calcium phosphates (CaPs). The in vitro dissolution behavior, antibacterial activity, and cytotoxicity of Ag-containing CaPs with different phase fractions of hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) were evaluated. The antibacterial activity of Ag-containing CaPs depended on the main phase of CaP, the chemical state of Ag, and the amount of incorporated Ag. Superior antibacterial activity was obtained from sustained release of Ag ions through continuous dissolution of Ag-incorporated β-TCP compared to that obtained for HA coexisting with metallic Ag particles. Ag-containing CaPs did not exhibit any toxic effect on V79 fibroblasts. Thus, these results demonstrated the effectiveness of Ag-incorporated β-TCP in preventing infection, with respect to long-term applications.
开发具有抗菌活性且无细胞毒性的生物陶瓷将有助于预防与植入物相关的感染。本研究旨在利用以金属形式掺入磷酸钙（CaP）中或与之共存的银（Ag）的抗菌特性。评估了具有不同羟基磷灰石（HA）和β-磷酸三钙（β-TCP）相分数的含Ag的CaP的体外溶解行为、抗菌活性和细胞毒性。含Ag的CaP的抗菌活性取决于CaP的主要相、Ag的化学状态以及掺入的Ag的量。与与金属Ag颗粒共存的HA相比，通过连续溶解掺入Ag的β-TCP持续释放Ag离子可获得更高的抗菌活性。含Ag的CaP对V79成纤维细胞未表现出任何毒性作用。因此，这些结果证明了掺入Ag的β-TCP在长期应用方面预防感染的有效性。
