Faculty of Mechanical Engineering and Ship Technology, Gdańsk University of Technology, 80-233 Gdansk, Poland.
Department of Biochemistry and Biotechnology, Medical University of Lublin, 20-093 Lublin, Poland.
Int J Mol Sci. 2021 Mar 20;22(6):3172. doi: 10.3390/ijms22063172.
The risk of an early inflammation after implantation surgery of titanium implants has caused the development of different antimicrobial measures. The present research is aimed at characterizing the effects of nanosilver and nanocopper dispersed in the nanohydroxyapatite coatings, deposited on the Ti13Zr13Nb alloy, and on the chemical and biological properties of the coatings. The one-stage deposition process was performed by the electrophoretic method at different contents of nanomaterials in suspension. The surface topography of the coatings was examined with scanning electron microscopy. The wettability was expressed as the water contact angle. The corrosion behavior was characterized by the potentiodynamic technique. The release rate of copper and silver into the simulated body fluid was investigated by atomic absorption spectrometry. The antibacterial efficiency was evaluated as the survivability and adhesion of the bacteria and the growth of the biofilm. The cytotoxicity was assessed for osteoblasts. The results demonstrate that silver and copper increase the corrosion resistance and hydrophilicity. Both elements together effectively kill bacteria and inhibit biofilm growth but appear to be toxic for osteoblasts. The obtained results show that the nanohydroxyapatite coatings doped with nanosilver and nanocopper in a one-stage electrophoretic process can be valuable for antibacterial coatings.
钛植入物植入手术后早期炎症的风险促使人们开发出不同的抗菌措施。本研究旨在表征纳米银和纳米铜在分散于纳米羟基磷灰石涂层中的作用，这些涂层沉积在 Ti13Zr13Nb 合金上，并研究涂层的化学和生物学性质。采用电泳法在悬浮液中不同纳米材料含量下进行一步沉积工艺。采用扫描电子显微镜检查涂层的表面形貌。润湿性表示为水接触角。通过动电位技术表征腐蚀行为。通过原子吸收光谱法研究铜和银在模拟体液中的释放速率。通过细菌的存活率和黏附性以及生物膜的生长来评估抗菌效率。评估成骨细胞的细胞毒性。结果表明，银和铜提高了耐腐蚀性和亲水性。两种元素共同有效地杀死细菌并抑制生物膜生长，但对成骨细胞似乎有毒。所得结果表明，在一步电泳工艺中掺杂纳米银和纳米铜的纳米羟基磷灰石涂层可能是有价值的抗菌涂层。
