Mai-Gisondi Galina, Master Emma R
Department of Bioproducts and Biosystems, Aalto University, 00076, Kemistintie 1, Espoo, Aalto, Finland.
Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College Street, Toronto, ON, M5S 3E5, Canada.
Methods Mol Biol. 2017;1588:45-57. doi: 10.1007/978-1-4939-6899-2_5.
Colorimetric detection of reaction products is typically preferred for initial surveys of acetyl xylan esterase (AcXE) activity. This chapter will describe common colorimetric methods, and variations thereof, for measuring AcXE activities on commercial, synthesized, and natural substrates. Whereas assays using pNP-acetate, α-naphthyl acetate, and 4-methylumbelliferyl acetate (4MUA) are emphasized, common methods used to measure AcXE activity towards carbohydrate analogs (e.g., acetylated p-nitrophenyl β-D-xylopyranosides) and various acetylated xylans are also described. Strengths and limitations of the colorimetric assays are highlighted.
对于乙酰木聚糖酯酶（AcXE）活性的初步检测，比色法检测反应产物通常更为可取。本章将描述用于测定商业、合成和天然底物上AcXE活性的常见比色法及其变体。重点介绍了使用对硝基苯乙酸酯、α-萘乙酸酯和4-甲基伞形酮乙酸酯（4MUA）的检测方法，同时也描述了用于测量AcXE对碳水化合物类似物（如乙酰化对硝基苯基β-D-吡喃木糖苷）和各种乙酰化木聚糖活性的常用方法。文中突出了比色法检测的优点和局限性。
