Biological Oceanography Division, CSIR-National Institute of Oceanography, Dona Paula, Goa, 403004, India.
Department of Microbiology, Goa University, Taleigao Plateau, Goa, 403206, India.
Bioprocess Biosyst Eng. 2021 Dec;44(12):2513-2524. doi: 10.1007/s00449-021-02623-6. Epub 2021 Aug 17.
A cellulase-free xylanolytic enzyme consortia consisting of a xylanase, arabinofuranosidase, and acetyl xylan esterase produced by Bacillus sp. NIORKP76 isolate under solid-state fermentation was assessed for its bio-bleaching ability on kraft pulp. In the biobleaching analysis, the xylanase dose of 5 Ug dry pulp denoted the optimum bleaching of pulp at 40 °C and pH 8.0 after 2 h of treatment. The reduction in kappa number of pre-treated hardwood pulp using xylanolytic enzyme consortium (XEC) was found to be ~ 55%, while solo xylanase could reduce the kappa number to 44-46%. In the case of chemical bagasse pulp, a reduction of ~ 27.5% and 19-20% was seen in kappa number using XEC and solo xylanase, respectively. Enzyme-treated pulp (HW and CB) showed a 50% reduction in hypochlorite consumption during the chlorine treatment. The current study results reveal the significant potential of xylanolytic enzyme consortium from Bacillus sp. NIORKP76 on the environmentally friendly bio-bleaching process.
一种由枯草芽孢杆菌产生的无纤维素酶木聚糖酶复合酶制剂，包括木聚糖酶、阿拉伯呋喃糖苷酶和乙酰木聚糖酯酶，在固态发酵下产生。该复合酶制剂在硫酸盐浆的生物漂白能力方面进行了评估。在生物漂白分析中，木聚糖酶用量为 5 Ug 干浆，在 40°C 和 pH8.0 下处理 2 小时后，达到最佳的纸浆漂白效果。使用木聚糖酶复合酶（XEC）预处理阔叶木浆，可使卡伯值降低约 55%，而单独使用木聚糖酶可将卡伯值降低至 44-46%。对于化学甘蔗渣浆，XEC 和单独的木聚糖酶分别可使卡伯值降低约 27.5%和 19-20%。经过酶处理的纸浆（HW 和 CB）在次氯酸盐处理过程中，可将次氯酸盐的消耗减少 50%。本研究结果表明，枯草芽孢杆菌的木聚糖酶复合酶制剂在环保生物漂白工艺方面具有显著的潜力。
