Nunes Cassiane da Silva Oliveira, de Carvalho Giovani Brandão Mafra, da Silva Marília Lordêlo Cardoso, da Silva Gervásio Paulo, Machado Bruna Aparecida Souza, Uetanabaro Ana Paula Trovatti
Department of Biology and Biotechnology of Microorganisms, State University of Santa Cruz, Ilhéus, Bahia, Brazil.
Bahia Federal Institute Catu Campus, Catu, Bahia, Brazil.
PLoS One. 2017 Apr 18;12(4):e0175677. doi: 10.1371/journal.pone.0175677. eCollection 2017.
This work evaluated the effect of cocoa pulp as a malt adjunct on the parameters of fermentation for beer production on a pilot scale. For this purpose, yeast isolated from the spontaneous fermentation of cachaça (SC52), belonging to the strain bank of the State University of Feira de Santana-Ba (Brazil), and a commercial strain of ale yeast (Safale S-04 Belgium) were used. The beer produced was subjected to acceptance and purchase intention tests for sensorial analysis. At the beginning of fermentation, 30% cocoa pulp (adjunct) was added to the wort at 12°P concentration. The production of beer on a pilot scale was carried out in a bioreactor with a 100-liter capacity, a usable volume of 60 liters, a temperature of 22°C and a fermentation time of 96 hours. The fermentation parameters evaluated were consumption of fermentable sugars and production of ethanol, glycerol and esters. The beer produced using the adjunct and yeast SC52 showed better fermentation performance and better acceptance according to sensorial analysis.
本研究在中试规模下评估了可可果肉作为麦芽辅料对啤酒生产发酵参数的影响。为此,使用了从巴西费拉德桑塔纳州立大学巴伊亚分校菌株库中分离的、用于卡莎萨自然发酵的酵母(SC52),以及一种商业艾尔酵母菌株(萨法乐S-04比利时)。对所生产的啤酒进行了感官分析的接受度和购买意愿测试。在发酵开始时,向浓度为12°P的麦芽汁中添加30%的可可果肉(辅料)。中试规模的啤酒生产在一个容量为100升、可用体积为60升、温度为22°C且发酵时间为96小时的生物反应器中进行。所评估的发酵参数包括可发酵糖的消耗以及乙醇、甘油和酯类的产生。根据感官分析,使用该辅料和酵母SC52生产的啤酒表现出更好的发酵性能和更高的接受度。