犬实验性脓毒性关节炎中的脂氧合酶产物

Lipoxygenase products in experimental septic arthritis in dogs.

作者信息

Herlin T, Fogh K, Knudsen V E, Hansen E S, Bunger E, Ewald H, Holm I, Kragballe K

机构信息

Department of Pediatrics, Aarhus Kommunehospital, Denmark.

出版信息

APMIS. 1988 Jul;96(7):596-600. doi: 10.1111/j.1699-0463.1988.tb00916.x.

DOI:10.1111/j.1699-0463.1988.tb00916.x

PMID:2841955

Abstract

Induction of an experimental septic arthritis was performed in the juvenile dog knee by an intra-articular injection with Staphylococcus aureus. From plasma, lipoxygenase (LO) products of arachidonic acid were separated by reversed-phase high-performance liquid chromatography (RP-HPLC) after extraction of lipids. The plasma samples did not contain UV-detectable amounts of LO products other than the LTB4 metabolite 20-COOH-LTB4, and 12-HETE. Small amounts of chemokinetically active material were found coeluting with the eluate fraction of 20-COOH-LTB4, LTB4 and 12-HETE after RP-HPLC. After 48 hours of septic arthritis, resulting in a massive acute joint inflammation, no significant changes in LO products were observed.

摘要

通过向幼犬膝关节内注射金黄色葡萄球菌诱导实验性脓毒性关节炎。脂质提取后，采用反相高效液相色谱（RP-HPLC）从血浆中分离花生四烯酸的脂氧合酶（LO）产物。血浆样本中除LTB4代谢物20-COOH-LTB4和12-HETE外，未含有紫外线可检测量的LO产物。RP-HPLC后发现少量趋化活性物质与20-COOH-LTB4、LTB4和12-HETE的洗脱部分共洗脱。脓毒性关节炎48小时后，导致大量急性关节炎症，未观察到LO产物有显著变化。