Zhou Aimin, Ma Hongping, Liu Enhui, Jiang Tongtong, Feng Shuang, Gong Shufang, Wang Jingang
College of Horticulture and Landscape Architecture, Northeast Agricultural University, Harbin 150030, China.
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2017 Apr 17;18(4):849. doi: 10.3390/ijms18040849.
, a perennial herbaceous flower and a member of the Caryophyllaceae family, has strong resistance to cold and drought stresses. To explore the transcriptional responses of to individual and combined stresses, we performed transcriptome sequencing of seedlings under normal conditions or subjected to cold treatment (CT), simulated drought treatment (DT), or their combination (CTDT). After de novo assembly of the obtained reads, 112,015 unigenes were generated. Analysis of differentially expressed genes (DEGs) showed that 2026, 940, and 2346 genes were up-regulated and 1468, 707, and 1759 were down-regulated in CT, DT, and CTDT samples, respectively. Among all the DEGs, 182 up-regulated and 116 down-regulated genes were identified in all the treatment groups. Analysis of metabolic pathways and regulatory networks associated with the DEGs revealed overlaps and cross-talk between cold and drought stress response pathways. The expression profiles of the selected DEGs in CT, DT, and CTDT samples were characterized and confirmed by quantitative RT-PCR. These DEGs and metabolic pathways may play important roles in the response of to the combined stress. Functional characterization of these genes and pathways will provide new targets for enhancement of plant stress tolerance through genetic manipulation.
[植物名称]是一种多年生草本花卉，属于石竹科，对寒冷和干旱胁迫具有较强的抗性。为了探究[植物名称]对单一胁迫和复合胁迫的转录响应，我们对正常条件下或经过冷处理（CT）、模拟干旱处理（DT）或其组合处理（CTDT）的幼苗进行了转录组测序。对获得的 reads 进行从头组装后，共生成了 112,015 个单基因。差异表达基因（DEG）分析表明，在 CT、DT 和 CTDT 样本中，分别有 2026、940 和 2346 个基因上调，1468、707 和 1759 个基因下调。在所有 DEG 中，在所有处理组中鉴定出 182 个上调基因和 116 个下调基因。对与 DEG 相关的代谢途径和调控网络的分析揭示了寒冷和干旱胁迫响应途径之间的重叠和相互作用。通过定量 RT-PCR 对 CT、DT 和 CTDT 样本中选定 DEG 的表达谱进行了表征和验证。这些 DEG 和代谢途径可能在[植物名称]对复合胁迫的响应中发挥重要作用。对这些基因和途径的功能表征将为通过基因操作提高植物胁迫耐受性提供新的靶点。
