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油茶转录组分析揭示了候选干旱胁迫基因。

Transcriptome analysis of the tea oil camellia (Camellia oleifera) reveals candidate drought stress genes.

作者信息

Dong Bin, Wu Bin, Hong Wenhong, Li Xiuping, Li Zhuo, Xue Li, Huang Yongfang

机构信息

South China Agricultural University, Guangzhou, Guangdong, China.

Guangdong Agriculture Industry Business Polytechnic, Guangzhou, Guangdong, China.

出版信息

PLoS One. 2017 Jul 31;12(7):e0181835. doi: 10.1371/journal.pone.0181835. eCollection 2017.

Abstract

BACKGROUND

The tea-oil camellia (Camellia oleifera) is the most important oil plant in southern China, and has a strong resistance to drought and barren soil. Understanding the molecular mechanisms of drought tolerance would greatly promote its cultivation and molecular breeding.

RESULTS

In total, we obtained 76,585 unigenes with an average length of 810 bp and an N50 of 1,092 bp. We mapped all the unigenes to the NCBI 'nr' (non-redundant), SwissProt, KEGG, and clusters of orthologous groups (COG) databases, where 52,531 (68.6%) unigenes were functionally annotated. According to the annotation, 46,171 (60.8%) unigenes belong to 338 KEGG pathways. We identified a series of unigenes that are related to the synthesis and regulation of abscisic acid (ABA), the activity of protective enzymes, vitamin B6 metabolism, the metabolism of osmolytes, and pathways related to the biosynthesis of secondary metabolites. After exposed to drought for 12 hours, the number of differentially-expressed genes (DEGs) between treated plants and control plants increased in the G4 cultivar, while there was no significant increase in the drought-tolerant C3 cultivar. DEGs associated with drought stress responsive pathways were identified by KEGG pathway enrichment analysis. Moreover, we found 789 DEGs related to transcription factors. Finally, according to the results of qRT-PCR, the expression levels of the 20 unigenes tested were consistent with the results of next-generation sequencing.

CONCLUSIONS

In the present study, we identified a large set of cDNA unigenes from C. oleifera annotated using public databases. Further studies of DEGs involved in metabolic pathways related to drought stress and transcription will facilitate the discovery of novel genes involved in resistance to drought stress in this commercially important plant.

摘要

背景

油茶是中国南方最重要的油料植物,对干旱和贫瘠土壤具有较强的抗性。了解其耐旱分子机制将极大地促进其栽培和分子育种。

结果

我们总共获得了76,585个单基因,平均长度为810 bp,N50为1,092 bp。我们将所有单基因映射到NCBI的“nr”(非冗余)、SwissProt、KEGG和直系同源基因簇(COG)数据库,其中52,531个(68.6%)单基因得到了功能注释。根据注释,46,171个(60.8%)单基因属于338条KEGG通路。我们鉴定出了一系列与脱落酸(ABA)合成与调控、保护酶活性、维生素B6代谢、渗透调节物质代谢以及次生代谢物生物合成相关的通路的单基因。在干旱处理12小时后,G4品种处理植株与对照植株之间的差异表达基因(DEG)数量增加,而耐旱的C3品种则没有显著增加。通过KEGG通路富集分析鉴定了与干旱胁迫响应通路相关的DEG。此外,我们发现了789个与转录因子相关的DEG。最后,根据qRT-PCR结果,所检测的20个单基因的表达水平与下一代测序结果一致。

结论

在本研究中,我们从油茶中鉴定出了大量使用公共数据库注释的cDNA单基因。对参与干旱胁迫相关代谢途径和转录的DEG的进一步研究将有助于发现这种具有重要商业价值的植物中参与耐旱胁迫的新基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d4cc/5536306/552d776083c2/pone.0181835.g001.jpg

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