Roman Brooke E, Driksna Dana, Abouzied Mohamed M, Klein Frank, Rice Jennifer
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912.
J AOAC Int. 2017 Jul 1;100(4):1131-1133. doi: 10.5740/jaoacint.17-0034. Epub 2017 Apr 18.
Neogen Corp. (Lansing, MI) has developed a common aqueous extraction method for the detection of mycotoxins in the ELISA or lateral flow format. The Veratox® for Total Aflatoxin ELISA extraction method uses a MAX 2 extraction packet and water in replacement of traditionally used organic solvents. Veratox for Total Aflatoxin has a detection range of 5-50 ppb neat or up to 300 ppb with dilution. The kit development focused on superior cross-reactivity, ability to accurately detect naturally contaminated samples, and utilization of an aqueous extraction method. In two separate validation studies, the Veratox for Total Aflatoxin test kit resulted in average yields of 91-114% in naturally contaminated mycotoxin reference material corn. The cross-reactivity profiles for aflatoxins B1, B2, G1, and G2 were 100, 113, 103, and 93%, respectively. This kit is approved by the Grain Inspection, Packers, and Stockyards Administration.
新奥金公司（密歇根州兰辛）开发了一种通用的水相萃取方法，用于以酶联免疫吸附测定（ELISA）或侧流免疫测定形式检测霉菌毒素。总黄曲霉毒素ELISA提取方法的Veratox®使用MAX 2提取包和水来替代传统使用的有机溶剂。总黄曲霉毒素的Veratox检测范围为纯品5 - 50 ppb，稀释后可达300 ppb。该试剂盒的开发重点在于卓越的交叉反应性、准确检测天然污染样品的能力以及水相萃取方法的应用。在两项独立的验证研究中，总黄曲霉毒素测试试剂盒在天然污染的霉菌毒素标准物质玉米中的平均回收率为91 - 114%。黄曲霉毒素B1、B2、G1和G2的交叉反应率分别为100%、113%、103%和93%。该试剂盒已获得谷物检验、包装和牲畜围场管理局的批准。
