Neogen Corporation, 620 Lesher Place, Lansing, MI, USA, 48912.
J AOAC Int. 2021 Mar 5;104(1):39-48. doi: 10.1093/jaoacint/qsaa113.
Many food recalls are related to the presence of undeclared allergens and microorganisms in food products. To reduce these occurrences, portable diagnostic assay kits are available to quantitate mycotoxins, to detect allergens and gluten in foods and on environmental surfaces, and for sanitation monitoring.
This article reviews diagnostic kits that can detect sources of contamination in food and ingredients as well as on surfaces and clean-in-place rinses.
Mycotoxins and gluten were detected using lateral flow diagnostic assays. Sanitation monitoring of surfaces was completed using a chemiluminescent assay to detect adenosine 5'-triphosphate disodium salt hydrate (ATP) and another assay to detect protein.
Gluten was detected at 10 ppm in spiked commodities and on wet and dry surfaces at 2.5 µg/100cm2. Deoxynivalenol was quantitated in dry distillers grains plus solubles and mean results were within two SDs of those determined by HPLC. The chemiluminescent assay had an LOD of 6 fmol of ATP and was able to detect a 1:10 000 dilution of orange juice from surfaces. The protein assay detected 5 µg of bovine serum albumin (BSA) directly applied to the sampler, 100 µg of BSA on surfaces, and detected 1:10 dilutions of Greek yogurt and raw beef from surfaces.
Portable diagnostic kits evaluated in this work provided accurate, rapid, and sensitive results for detection of mycotoxins, gluten, proteins, and ATP. These methods can be used in facilities with minimal training and provide results that are important to ensure food safety.
Portable methods to detect gluten, mycotoxins, proteins, and ATP are presented.
许多食品召回事件都与食品产品中未申报的过敏原和微生物有关。为了减少这些情况的发生，现在有便携式诊断检测试剂盒可用于定量检测真菌毒素，检测食品及环境表面中的过敏原和麸质，以及进行卫生监测。
本文综述了可用于检测食品和配料中以及表面和就地清洗冲洗物中污染来源的诊断试剂盒。
使用侧向流动诊断检测法检测真菌毒素和麸质。使用化学发光法检测腺苷 5'-三磷酸二钠盐水合物 (ATP) 和另一种检测蛋白质的方法来完成表面卫生监测。
在加标商品以及潮湿和干燥表面上，可检测到浓度为 10ppm 的麸质，检测限为 2.5µg/100cm2。在干酒糟及其可溶物中定量检测到脱氧雪腐镰刀菌烯醇，其平均结果与 HPLC 测定结果相差两个标准差以内。化学发光法的检测限为 6 飞摩尔的 ATP，能够检测到表面橙汁的 1:10000 稀释液。蛋白质检测法可直接检测到采样器上的 5µg 牛血清白蛋白 (BSA)、表面上的 100µg BSA，以及从表面检测到希腊酸奶和生牛肉的 1:10 稀释液。
本文评估的便携式诊断试剂盒为检测真菌毒素、麸质、蛋白质和 ATP 提供了准确、快速和灵敏的结果。这些方法可以在经过最少培训的设施中使用，并提供对确保食品安全很重要的结果。
本文介绍了用于检测麸质、真菌毒素、蛋白质和 ATP 的便携式方法。
