Lupo Anthony, Roebuck Chris, Dutcher Monica, Kennedy Justina, Abouzied Mohamed
Neogen Corp., 620 Lesher Pl, Lansing, MI 48912, USA.
J AOAC Int. 2010 Mar-Apr;93(2):587-99.
Neogen Corp. developed the Veratox aflatoxin test kit for the detection of total aflatoxin. The purpose of this study was to validate the method under the requirements of the AOAC Research Institute Performance Tested Methods (PTM) program. There are several AOAC Official Methods for total aflatoxin detection in corn (994.08, 990.33, 979.18, 993.17, 990.32, 993.16, 991.31, and 990.74), varying between rapid and analytical-based methods and one rapid method that has been performance tested by the AOAC Research Institute (PTM 030701). However, the widely used reference method is AOAC Official Method 994.08, which is an HPLC method and is referred to as the reference method in this paper. Although considered the reference method, the HPLC procedure is complicated and requires the investment of both expensive equipment and a highly skilled technician. A rapid (e.g., ELISA) test kit to be validated by the AOAC Research Institute is needed.
Neogen公司开发了用于检测总黄曲霉毒素的Veratox黄曲霉毒素检测试剂盒。本研究的目的是在AOAC研究机构性能验证方法（PTM）计划的要求下验证该方法。有几种AOAC官方方法用于玉米中总黄曲霉毒素的检测（994.08、990.33、979.18、993.17、990.32、993.16、991.31和990.74），这些方法在快速方法和基于分析的方法之间有所不同，还有一种快速方法已由AOAC研究机构进行了性能验证（PTM 030701）。然而，广泛使用的参考方法是AOAC官方方法994.08，这是一种HPLC方法，在本文中被称为参考方法。尽管被视为参考方法，但HPLC程序复杂，需要投入昂贵的设备和高技能的技术人员。因此，需要一种由AOAC研究机构验证的快速（例如ELISA）检测试剂盒。
