Rizzino A, Kuszynski C, Ruff E, Tiesman J
Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha 68105.
Dev Biol. 1988 Sep;129(1):61-71. doi: 10.1016/0012-1606(88)90161-3.
Previous studies have established that embryonal carcinoma (EC) cells produce several different growth factors, but express few, if any, receptors for epidermal growth factor, platelet-derived growth factor, or transforming growth factor type-beta. In this study, the production and utilization of fibroblast growth factor (FGF) by EC cells and their differentiated cells were investigated. We have determined that EC cells produce a heat-labile, heparin-binding factor that competes with FGF for binding to membrane receptors and appears to be immunologically related to FGF. The same or a similar factor is produced by three different EC cell lines, including a multipotent human EC cell line. However, production of this factor is apparently reduced when each EC cell line differentiates. Unlike the parental EC cells, the differentiated cells respond to FGF by growth stimulation and the growth responses to FGF correlate with increased binding of FGF. Although the binding data indicate that both the EC cells and their differentiated cells exhibit high affinity receptors for FGF, the differentiated cells express these receptors at levels approximately 10-fold higher. These findings suggest that the FGF-related growth factor could influence the growth of EC cells or their differentiated cells.
以往的研究已证实，胚胎癌（EC）细胞能产生几种不同的生长因子，但几乎不表达（即便有也极少）表皮生长因子、血小板衍生生长因子或β型转化生长因子的受体。在本研究中，对EC细胞及其分化细胞产生和利用成纤维细胞生长因子（FGF）的情况进行了调查。我们已确定，EC细胞能产生一种不耐热的、与肝素结合的因子，该因子与FGF竞争结合膜受体，且在免疫学上似乎与FGF相关。三种不同的EC细胞系，包括一种多能人类EC细胞系，都会产生相同或相似的因子。然而，当每个EC细胞系分化时，这种因子的产生明显减少。与亲代EC细胞不同，分化细胞对FGF有生长刺激反应，且对FGF的生长反应与FGF结合增加相关。尽管结合数据表明EC细胞及其分化细胞都表现出对FGF的高亲和力受体，但分化细胞表达这些受体的水平大约高10倍。这些发现表明，FGF相关生长因子可能会影响EC细胞或其分化细胞的生长。
