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用于潜在生物医学应用的亚 5nm 硅涂层磁性氧化铁荧光纳米粒子的体外生物相容性研究。

In vitro biocompatibility study of sub-5 nm silica-coated magnetic iron oxide fluorescent nanoparticles for potential biomedical application.

机构信息

Institute of Materials for Electronics and Magnetism (IMEM), Department of Engineering, ICT and technologies for energy and transportation, National Research Council (CNR), Parma, Italy.

Institute of Translational Pharmacology (IFT), Department of Biomedical Sciences, National Research Council (CNR), Rome, Italy.

出版信息

Sci Rep. 2017 Apr 19;7:46513. doi: 10.1038/srep46513.

Abstract

Magnetic iron oxide nanoparticles (IONPs), for their intriguing properties, have attracted a great interest as they can be employed in many different biomedical applications. In this multidisciplinary study, we synthetized and characterized ultrafine 3 nm superparamagnetic water-dispersible nanoparticles. By a facile and inexpensive one-pot approach, nanoparticles were coated with a shell of silica and contemporarily functionalized with fluorescein isothiocyanate (FITC) dye. The obtained sub-5 nm silica-coated magnetic iron oxide fluorescent (sub-5 SIO-Fl) nanoparticles were assayed for cellular uptake, biocompatibility and cytotoxicity in a human colon cancer cellular model. By confocal microscopy analysis we demonstrated that nanoparticles as-synthesized are internalized and do not interfere with the CaCo-2 cell cytoskeletal organization nor with their cellular adhesion. We assessed that they do not exhibit cytotoxicity, providing evidence that they do not affect shape, proliferation, cellular viability, cell cycle distribution and progression. We further demonstrated at molecular level that these nanoparticles do not interfere with the expression of key differentiation markers and do not affect pro-inflammatory cytokines response in Caco-2 cells. Overall, these results showed the in vitro biocompatibility of the sub-5 SIO-Fl nanoparticles promising their safe employ for diagnostic and therapeutic biomedical applications.

摘要

磁性氧化铁纳米粒子(IONPs)因其独特的性质而备受关注,因为它们可以应用于许多不同的生物医学领域。在这项多学科研究中,我们合成并表征了超小的 3nm 超顺磁水分散纳米粒子。通过简便、廉价的一锅法,我们用二氧化硅壳层对纳米粒子进行了包覆,并同时用异硫氰酸荧光素(FITC)染料对其进行了功能化。所获得的亚 5nm 二氧化硅包覆的磁性氧化铁荧光(sub-5 SIO-Fl)纳米粒子在人结肠癌细胞模型中进行了细胞摄取、生物相容性和细胞毒性的检测。通过共聚焦显微镜分析,我们证明了所合成的纳米粒子可以被内吞,并且不会干扰 CaCo-2 细胞的细胞骨架组织,也不会影响其细胞黏附。我们评估了它们没有表现出细胞毒性,这表明它们不会影响细胞的形态、增殖、细胞活力、细胞周期分布和进展。我们还在分子水平上证明了这些纳米粒子不会干扰关键分化标记物的表达,也不会影响 Caco-2 细胞中促炎细胞因子的反应。总的来说,这些结果表明 sub-5 SIO-Fl 纳米粒子具有良好的体外生物相容性,有望安全地应用于诊断和治疗生物医学领域。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/93d4/5395943/13a9deac2c71/srep46513-f1.jpg

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