Mitrani-Rosenbaum S
Department of Virology, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Intervirology. 1988;29(2):108-14. doi: 10.1159/000150035.
The human beta interferon (hIFN beta 1) gene has been cloned into a bovine papillomavirus type 1 (BPV-1)/pML2 vector. The recombinant DNAs can be maintained as stable plasmids in mouse cells, with no detectable rearrangements. The linked hIFN beta 1 gene is inducible with little line-to-line variability in independently transformed cell lines. The hIFN beta 1 gene is equally induced when it is inserted adjacent to or away from the BPV-1 enhancer. The great stability of the BPV-1/pML2 vector has allowed us to study the regulation of the hIFN beta 1 gene in mouse cells, with the construction of deleted and hybrid hIFN beta 1 genes. Using this strategy, we have confirmed that the 5' sequences of the hIFN beta 1 gene are involved in the induction mechanism. The entire hIFN beta 1 regulatory element is able to express heterologous genes with no induction, suggesting that the negative regulatory sequences present in the 3' region of the hIFN beta 1 regulatory element are not fully active on heterologous genes.
人β干扰素（hIFNβ1）基因已被克隆到1型牛乳头瘤病毒（BPV-1）/pML2载体中。重组DNA能作为稳定的质粒在小鼠细胞中维持，未检测到重排。在独立转化的细胞系中，连接的hIFNβ1基因可被诱导，且细胞系间的变异性很小。当hIFNβ1基因插入到BPV-1增强子附近或远离该增强子时，其诱导情况相同。BPV-1/pML2载体的高度稳定性使我们能够通过构建缺失和杂交的hIFNβ1基因来研究小鼠细胞中hIFNβ1基因的调控。利用这一策略，我们证实了hIFNβ1基因的5'序列参与诱导机制。整个hIFNβ1调控元件能够在无诱导的情况下表达异源基因，这表明hIFNβ1调控元件3'区域中存在的负调控序列对异源基因的作用并不完全有效。
