Jishma P, Hussain N, Chellappan R, Rajendran R, Mathew J, Radhakrishnan E K
School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India.
J Appl Microbiol. 2017 Jul;123(1):204-216. doi: 10.1111/jam.13474.
Analysis of a blend of volatile organic compounds (VOCs) released by Pseudomonas spp. with growth-promoting effect in Vigna radiata seedlings.
Plant growth-promoting activity of VOCs produced by five different Pseudomonas spp. was investigated by I-plate technique. VOCs produced by different organisms were extracted by various solvents (methanol, ethyl acetate, hexane and butanol) and were identified by gas chromatography-mass spectrometric analysis. The major VOCs identified were undecane, nonadecane, hexacosane, tetradecane, heptacosane, pentadecane, dodecane and tetratetracontane. The cultural conditions for the production of VOCs with plant growth enhancement effect were optimized and further confirmed using pure pentadecane as a candidate VOC.
The study provides insight into plant beneficial effect of VOCs produced by Pseudomonas spp. Remarkable modulation in the production of VOCs with plant growth-promoting effect by rhizobacteria was found to be dependent on the bacterial strain and its concentration.
The study describes the requirement to consider bacterial VOC production also for preparing plant probiotic formulations. As VOCs are least considered for preparation of commercial plant growth-promoting rhizobacterial strain formulation, the results of the study is highly significant.
分析具有促进绿豆幼苗生长作用的假单胞菌属释放的挥发性有机化合物（VOCs）混合物。
采用I-平板技术研究了五种不同假单胞菌属产生的VOCs的植物促生活性。不同菌株产生的VOCs用多种溶剂（甲醇、乙酸乙酯、己烷和丁醇）进行提取，并通过气相色谱-质谱分析进行鉴定。鉴定出的主要VOCs为十一烷、十九烷、二十六烷、十四烷、二十七烷、十五烷、十二烷和四十四烷。优化了具有促进植物生长作用的VOCs的生产培养条件，并以纯十五烷作为候选VOC进行了进一步验证。
该研究深入了解了假单胞菌属产生的VOCs对植物的有益作用。发现根际细菌产生的具有促进植物生长作用的VOCs的显著调节取决于细菌菌株及其浓度。
该研究描述了在制备植物益生菌制剂时也需要考虑细菌VOC产生的情况。由于在商业植物促生根际细菌菌株制剂的制备中最少考虑VOCs，该研究结果具有高度重要性。
