Arteaga-Ríos Itzel G, Méndez-Rodríguez Karen Beatriz, Ocampo-Pérez Raul, Guerrero-González María de la Luz, Rodríguez-Guerra Raúl, Delgado-Sánchez Pablo
Facultad de Agronomía y Veterinaria. Universidad Autónoma de San Luis Potosí. Soledad de Graciano Sánchez, SLP, CP, 78321. México.
Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología, Universidad Autónoma de San Luis Potosí, San Luis Potosí, SLP, CP, 78210, México.
Curr Res Microb Sci. 2024 Nov 13;8:100312. doi: 10.1016/j.crmicr.2024.100312. eCollection 2025.
Currently, the use of bio-inputs is increasing due to the need to reduce the use of agrochemicals. However, one of the limitations is to preserve the viability of the living microorganisms, so it is important to find an alternative that allows us to obtain different metabolites to produce it. We evaluated three different interactions (contact, diffusible and volatile compounds) in (At) seedlings with the strain M10 and its filtered secondary metabolites (M10F). The results showed that the seedlings inoculated by contact with the filtrate (AtM10F) presented increases in root length (30 %) and leaf area (33 %), as well as in the volatile interaction (At/M10F) with respect to the uninoculated treatment. For both interactions, the seedlings inoculated with the bacteria by contact (AtM10) and volatile (At/M10) obtained greater biomass (48 and 57 %). Subsequently, an evaluation at the end of the cycle showed that the treatments obtained by contact and distance when reinoculated with the bacteria and the filtrate (AtM10, At-M10 and AtM10F) obtained 50 % more seed yield than the control treatment, while AtM10F presented 72 %, while At/M10F presented the highest no. of siliques and seeds, which increased the yield by 65 %. In the (Sl) experiment, the filtrate (SlM10F) showed significant differences in seedling height, leaf length and width (23, 24 and 36 %, respectively). It also promoted an increase in fresh and dry weight, producing a greater root area and larger leaves compared to the control (Sl) and the bacteria (SlM10). We performed a qualitative characterization of the secondary metabolites present in the filtrate, where we found 2,4-DTBP, sylvopinol, isophthaladehyde, and eicosane of interest with possible growth-promoting effects on and tomato. We identified volatile compounds present in plant-microorganism and plant-filtrate interactions as possible precursors in the induction of plant growth, among which phenols, alcohols, aldehydes, alkanes, and alkenes stand out. Most of the analyzed compounds have not been found in the literature with reports of growth promoters, is important to mention that due to their characteristic functional groups they can derive and trigger the synthesis of new molecules with agronomic application.
目前，由于需要减少农用化学品的使用，生物投入物的使用正在增加。然而，其中一个限制是要保持活微生物的活力，因此找到一种能让我们获得不同代谢物以进行生产的替代方法很重要。我们评估了拟南芥（At）幼苗与M10菌株及其过滤后的次生代谢产物（M10F）之间的三种不同相互作用（接触、可扩散和挥发性化合物）。结果表明，与滤液接触接种的幼苗（AtM10F）的根长（增加30%）和叶面积（增加33%），以及与未接种处理相比的挥发性相互作用（At/M10F）均有所增加。对于这两种相互作用，通过接触接种细菌的幼苗（AtM10）和挥发性接种的幼苗（At/M10）获得了更大的生物量（分别为48%和57%）。随后，在周期结束时的评估表明，当再次接种细菌和滤液时，通过接触和远距离获得的处理（AtM10、At - M10和AtM10F）的种子产量比对照处理高出50%，而AtM10F的种子产量为72%，而At/M10F的角果和种子数量最多，产量提高了65%。在番茄（Sl）实验中，滤液（SlM10F）在幼苗高度、叶长和叶宽方面表现出显著差异（分别为23%、24%和36%）。它还促进了鲜重和干重的增加，与对照（Sl）和细菌（SlM10）相比，产生了更大的根面积和更大的叶子。我们对滤液中存在的次生代谢产物进行了定性表征，发现了2,4 - DTBP、sylvopinol、间苯二甲醛和二十烷，这些物质可能对拟南芥和番茄具有促进生长的作用。我们确定了植物 - 微生物和植物 - 滤液相互作用中存在的挥发性化合物可能是诱导植物生长的前体，其中酚类、醇类、醛类、烷烃和烯烃尤为突出。大多数分析的化合物在文献中未被报道具有生长促进作用，需要提及的是，由于它们的特征官能团，它们可以衍生并引发具有农艺应用的新分子的合成。
