Dong Zhihao, Yuan Xianjun, Wen Aiyou, Desta Seare T, Shao Tao
Institute of Ensiling and Processing of Grass, Nanjing Agricultural University, Nanjing 210095, China.
College of Animal Science, Anhui Science and Technology University, Fengyang 233100, China.
Asian-Australas J Anim Sci. 2017 Sep;30(9):1278-1284. doi: 10.5713/ajas.16.0956. Epub 2017 Mar 25.
To assess the potency of calcium propionate (CAP) used as silage additive, an experiment was carried out to evaluate the effect of CAP on the nitrogen transformation, fermentation quality and aerobic stability of alfalfa silages.
Alfalfa was ensiled with four levels of CAP (5, 10, 15, and 20 g/kg of fresh weight [FW]) in laboratory silos for 30 days. After opening, the silages were analyzed for the chemical and microbiological characteristics, and subjected to an aerobic stability test.
The increasing proportion of CAP did not affect pH, lactic acid (LA) concentrations and yeast counts, while linearly decreased counts of enterobacteria (p = 0.029), molds (p<0.001) and clostridia (p<0.001), and concentrations of acetic acid (p<0.001), propionic acid (p<0.001), butyric acid (p<0.001), and ethanol (p = 0.007), and quadratically (p = 0.001) increased lactic acid bacteria counts. With increasing the proportion of CAP, the dry matter (DM) loss (p<0.001), free amino acid N (p<0.001), ammonia N (p = 0.004), and non-protein N (p<0.001) contents were linearly reduced, whereas DM (p = 0.048), water soluble carbohydrate (p<0.001) and peptide N (p<0.001) contents were linearly increased. The highest Flieg's point was found in CAP10 (75.9), represented the best fermentation quality. All silages treated with CAP improved aerobic stability as indicated by increased stable hours compared with control.
The addition of CAP can suppress the undesirable microorganisms during ensiling and exposure to air, thereby improving the fermentation quality and aerobic stability as well as retarding the proteolysis of alfalfa silage. It is suggested that CAP used as an additive is recommended at a level of 10 g/kg FW.
为评估丙酸钙(CAP)作为青贮添加剂的效能,开展了一项实验以评价CAP对苜蓿青贮饲料氮转化、发酵品质及有氧稳定性的影响。
在实验室青贮窖中,将苜蓿与四个水平的CAP(5、10、15和20克/千克鲜重[FW])一起青贮30天。开封后,分析青贮饲料的化学和微生物特性,并进行有氧稳定性测试。
CAP比例增加未影响pH值、乳酸(LA)浓度和酵母计数,但肠杆菌计数(p = 0.029)、霉菌计数(p<0.001)和梭菌计数(p<0.001)呈线性下降(p<0.001),乙酸浓度(p<0.001)、丙酸浓度(p<0.001)、丁酸浓度(p<0.001)和乙醇浓度(p = 0.007)也呈线性下降,而乳酸菌计数呈二次方增加(p = 0.001)。随着CAP比例增加干物质(DM)损失(p<0.001)、游离氨基酸氮(p<0.001)、氨氮(p = 0.004)和非蛋白氮(p<0.001)含量呈线性降低,而DM(p = 0.048)、水溶性碳水化合物(p<0.001)和肽氮(p<0.001)含量呈线性增加CAP10的弗利格点最高(75.9),表明发酵品质最佳。与对照相比,所有用CAP处理的青贮饲料稳定时长增加,表明有氧稳定性得到改善。
添加CAP可在青贮及暴露于空气期间抑制不良微生物,从而改善发酵品质及有氧稳定性,并延缓苜蓿青贮饲料的蛋白水解作用建议CAP作为添加剂的推荐用量为10克/千克FW。