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利用微卫星标记对韩国(大邱市新川河)欧亚水獭(Lutra lutra)进行个体识别

Individual identification of Eurasian otters (Lutra lutra) in South Korea (Sincheon River, Daegu) by microsatellite markers.

作者信息

Park Chul-Song, Cho Gil-Jae

机构信息

College of Veterinary Medicine and Institute of Equine Medicine, Kyungpook National University, Daegu 41566, South Korea.

出版信息

J Vet Med Sci. 2017 Jun 16;79(6):1064-1067. doi: 10.1292/jvms.16-0563. Epub 2017 Apr 20.

DOI:10.1292/jvms.16-0563
PMID:28428484
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5487784/
Abstract

The objective of this study was to determine the number of Eurasian otters (Lutra lutra) that occupied the Sincheon River in Daegu, South Korea. Twenty-seven spraints collected from February to May 2016 at four sites (Jangam Bridge approximately 6.1 km from the Gachang Dam, Docheong Bridge approximately 13.5 km, Chimsan Bridge approximately 15.1 km and Nogoek Bridge approximately 18 km) along the Sincheon River (approximately 27.06 km) were analyzed using 12 microsatellite markers. The analyses resulted in the identification of 16 (59.3%) individual Eurasian otters in the Sincheon River based on the 27 spraints. Of the 16 individual Eurasian otters, seven were male, and nine were female. Groups were centered at the Jangam Bridge (3 males and 2 females), Chimsan Bridge (2 males and 3 females) and Docheong Bridge (2 males and 4 females). Thus, the 16 Eurasian otters formed three genetically related groups in each sampling area. The number of alleles per locus varied from three to seven, with a mean value of 5.08 alleles.

摘要

本研究的目的是确定占据韩国大邱市新川河的欧亚水獭(Lutra lutra)数量。2016年2月至5月期间,在新川河(约27.06公里)沿线的四个地点(距加昌大坝约6.1公里的长岩桥、约13.5公里的道清桥、约15.1公里的 Chim-san桥和约18公里的野谷桥)收集了27份粪便样本,使用12个微卫星标记进行分析。分析结果显示,基于这27份粪便样本,在新川河中识别出了16只(59.3%)欧亚水獭个体。在这16只欧亚水獭个体中,7只为雄性,9只为雌性。群体集中在长岩桥(3只雄性和2只雌性)、Chim-san桥(2只雄性和3只雌性)和道清桥(2只雄性和4只雌性)。因此,这16只欧亚水獭在每个采样区域形成了三个遗传相关的群体。每个位点的等位基因数量从3个到7个不等,平均值为5.08个等位基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8534/5487784/c6a99d8fb969/jvms-79-1064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8534/5487784/ef22180faf84/jvms-79-1064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8534/5487784/c6a99d8fb969/jvms-79-1064-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8534/5487784/ef22180faf84/jvms-79-1064-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8534/5487784/c6a99d8fb969/jvms-79-1064-g002.jpg

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