Alford R L, Hammond H A, Coto I, Caskey C T
Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030.
Am J Hum Genet. 1994 Jul;55(1):190-5.
Short tandem repeat (STR) loci are highly informative polymorphic loci that are gaining popularity for identity testing. We have conducted parentage testing by using nine STR loci on 50 paternity trios that had been previously tested using VNTR loci. These nine unlinked STR loci are amplified in three multiplex reactions and, when examined for genetic informativeness, provide a combined average power of exclusion of 99.73% (Caucasian data). The informative value of the selected loci is based on extensive STR typing of four racial/ethnic populations. In 37 of the 50 cases, paternity could not be excluded by any of the loci. In the remaining 13 cases, paternity was excluded by at least two of the STR markers. The probability of paternity calculated for the alleged father of each matching trio was > 99% in 36 of the 37 inclusion cases. All data agreed with the results reported using VNTR loci and conventional Southern technology. Our studies validate the use of DNA typing with STR loci for parentage testing, thus providing an accurate, highly sensitive, and rapid assay.
短串联重复序列（STR）位点是高度信息丰富的多态性位点，在身份鉴定测试中越来越受欢迎。我们使用9个STR位点对50个亲权三联体进行了亲权测试，这些三联体之前已使用可变数目串联重复序列（VNTR）位点进行过测试。这9个不连锁的STR位点在三个多重反应中进行扩增，在检测遗传信息时，其综合平均排除概率为99.73%（高加索人数据）。所选位点的信息价值基于对四个种族/民族群体进行的广泛STR分型。在50个案例中的37个案例中，任何位点都不能排除亲权。在其余13个案例中，至少有两个STR标记排除了亲权。在37个包含案例中的36个案例中，为每个匹配三联体的推定父亲计算的亲权概率>99%。所有数据与使用VNTR位点和传统Southern技术报告的结果一致。我们的研究验证了使用STR位点进行DNA分型用于亲权测试的有效性，从而提供了一种准确、高度灵敏且快速的检测方法。
