Petrenko V A, Pakharukova M Yu, Kovner A V, L'vova M N, Lyakhovich V V, Mordvinov V A
Federal Research Centre Institute of Cytology and Genetics of the Siberian Division of the Russian Academy of Sciences, Novosibirsk, Russia.
Research Institute of Molecular Biology and Biophysics, Novosibirsk, Russia.
Bull Exp Biol Med. 2017 Apr;162(6):773-776. doi: 10.1007/s10517-017-3710-5. Epub 2017 Apr 21.
Mechanisms of thioredoxin peroxidase secretion by Opisthorchis felineus were studied in vivo and in vitro. Specific antibodies were obtained and used for western blotting and immunohistochemical detection in Syrian hamster model of opisthorchiasis. Secreted thioredoxin peroxidase protein was accumulated in the worm incubation medium under conditions of oxidative stress and in bile duct cells of hamsters with chronic opisthorchiasis.
在体内和体外研究了猫后睾吸虫分泌硫氧还蛋白过氧化物酶的机制。制备了特异性抗体，并将其用于后睾吸虫病叙利亚仓鼠模型的蛋白质免疫印迹和免疫组织化学检测。在氧化应激条件下，分泌的硫氧还蛋白过氧化物酶蛋白在蠕虫培养液中蓄积，并且在慢性后睾吸虫病仓鼠的胆管细胞中也有蓄积。
